@article{1e096f50e8be4a35a0af0ce625b8c63c,
title = "Detection of vulnerable neurons damaged by environmental insults in utero",
abstract = "Development of prognostic biomarkers for the detection of prenatally damaged neurons before manifestations of postnatal disorders is an essential step for prevention and treatment of susceptible individuals. We have developed a versatile fluorescence reporter system in mice enabling detection of Heat Shock Factor 1 activation in response to prenatal cellular damage caused by exposure to various harmful chemical or physical agents. Using an intrautero electroporationmediated reporter assay and transgenic reporter mice, we are able to identify neurons that survive prenatal exposure to harmful agents but remain vulnerable in postnatal life. This system may provide a powerful tool for exploring the pathogenesis and treatment of multiple disorders caused by exposure to environmental stress before symptoms become manifested, exacerbated, and/or irreversible.",
keywords = "Cerebral cortex, Development, Environmental stress, Heat shock signaling, Reporter",
author = "Masaaki Torii and Masanori Sasaki and Chang, {Yu Wen} and Seiji Ishii and Waxman, {Stephen G.} and Kocsis, {Jeffery D.} and Pasko Rakic and Kazue Hashimoto-Torii",
note = "Funding Information: We thank Dr. Alexander Son for critical reading of the manuscript. This work was supported by National Institute of Health Grants R21AA024882 (to K.H.-T. and M.T.), R01AA025215 (to K.H.-T.), R01DA023999 and R01EY002593 (to P.R.), the Alcoholic Beverage Medical Research Foundation/The Foundation for Alcohol Research and Clinical and Translational Science Institute at Children's National (K.H.-T.); the Brain and Behavior Research Foundation, Scott-Gentle Foundation, and the Kavli Institute for Neuroscience at Yale (K.H.-T., M.T., and P.R.); Avery Translational Research Career Development Program Award (M.T.); Japan Society for the Promotion of Science KAKENHI Grants 24890181 and 25462227 (to M.S.); the Medical and Rehabilitation and Development Research Services of Department of Veterans Affairs (S.G.W.); and the VA Spinal Cord Injury Translational Collaborative Consortium for Regenerative Medicine, National Multiple Sclerosis Society Grant RG2135, and Connecticut's Stem Cell Research Program 12-SCB-Yale-05 (to J.D.K.). Publisher Copyright: {\textcopyright} 2017, National Academy of Sciences. All rights reserved.",
year = "2017",
month = feb,
day = "28",
doi = "10.1073/pnas.1620641114",
language = "English",
volume = "114",
pages = "2367--2372",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "9",
}