Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass

Hideko Kanazawa, Yoshiko Nagata, Yoshikazu Matsushima, Masashi Tomoda, Nobuharu Takai

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20 Citations (Scopus)

Abstract

High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2 PO4 as the mobile phase at room temperature.

Original languageEnglish
Pages (from-to)408-414
Number of pages7
JournalJournal of Chromatography A
Volume630
Issue number1-2
DOIs
Publication statusPublished - 1993 Feb 5

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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