Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass

Hideko Kanazawa, Yoshiko Itoh, Yoshikazu Matsushima, Masashi Tomoda, Nobuharu Takai

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2 PO4 as the mobile phase at room temperature.

Original languageEnglish
Pages (from-to)408-414
Number of pages7
JournalJournal of Chromatography A
Volume630
Issue number1-2
DOIs
Publication statusPublished - 1993 Feb 5

Fingerprint

Saponins
High performance liquid chromatography
Glass
High Pressure Liquid Chromatography
Bupleurum
Pharmaceutical Preparations
Ginsenosides
Panax
Temperature
Liquids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass. / Kanazawa, Hideko; Itoh, Yoshiko; Matsushima, Yoshikazu; Tomoda, Masashi; Takai, Nobuharu.

In: Journal of Chromatography A, Vol. 630, No. 1-2, 05.02.1993, p. 408-414.

Research output: Contribution to journalArticle

@article{b751024603154b37a18797e057d9429b,
title = "Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass",
abstract = "High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2 PO4 as the mobile phase at room temperature.",
author = "Hideko Kanazawa and Yoshiko Itoh and Yoshikazu Matsushima and Masashi Tomoda and Nobuharu Takai",
year = "1993",
month = "2",
day = "5",
doi = "10.1016/0021-9673(93)80479-R",
language = "English",
volume = "630",
pages = "408--414",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass

AU - Kanazawa, Hideko

AU - Itoh, Yoshiko

AU - Matsushima, Yoshikazu

AU - Tomoda, Masashi

AU - Takai, Nobuharu

PY - 1993/2/5

Y1 - 1993/2/5

N2 - High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2 PO4 as the mobile phase at room temperature.

AB - High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2 PO4 as the mobile phase at room temperature.

UR - http://www.scopus.com/inward/record.url?scp=0027476897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027476897&partnerID=8YFLogxK

U2 - 10.1016/0021-9673(93)80479-R

DO - 10.1016/0021-9673(93)80479-R

M3 - Article

C2 - 8445006

AN - SCOPUS:0027476897

VL - 630

SP - 408

EP - 414

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1-2

ER -