Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass

Hideko Kanazawa; Yoshiko Nagata; Yoshikazu Matsushima; Masashi Tomoda; Nobuharu Takai

doi:10.1016/0021-9673(93)80479-R

Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass

Hideko Kanazawa, Yoshiko Nagata, Yoshikazu Matsushima, Masashi Tomoda, Nobuharu Takai

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article

20 Citations (Scopus)

Abstract

High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH₂ PO₄ as the mobile phase at room temperature.

Original language	English
Pages (from-to)	408-414
Number of pages	7
Journal	Journal of Chromatography A
Volume	630
Issue number	1-2
DOIs	https://doi.org/10.1016/0021-9673(93)80479-R
Publication status	Published - 1993 Feb 5

Fingerprint

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

Cite this

Kanazawa, H., Nagata, Y., Matsushima, Y., Tomoda, M., & Takai, N. (1993). Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass. Journal of Chromatography A, 630(1-2), 408-414. https://doi.org/10.1016/0021-9673(93)80479-R

@article{b751024603154b37a18797e057d9429b,

title = "Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass",

abstract = "High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2 PO4 as the mobile phase at room temperature.",

author = "Hideko Kanazawa and Yoshiko Nagata and Yoshikazu Matsushima and Masashi Tomoda and Nobuharu Takai",

year = "1993",

month = feb,

day = "5",

doi = "10.1016/0021-9673(93)80479-R",

language = "English",

volume = "630",

pages = "408--414",

journal = "Journal of Chromatography A",

issn = "0021-9673",

number = "1-2",

}

TY - JOUR

T1 - Determination of acidic saponins in crude drugs by high- performance liquid chromatography on octadecylsilyl porous glass

AU - Kanazawa, Hideko

AU - Nagata, Yoshiko

AU - Matsushima, Yoshikazu

AU - Tomoda, Masashi

AU - Takai, Nobuharu

PY - 1993/2/5

Y1 - 1993/2/5

N2 - High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2 PO4 as the mobile phase at room temperature.

AB - High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2 PO4 as the mobile phase at room temperature.

UR - http://www.scopus.com/inward/record.url?scp=0027476897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027476897&partnerID=8YFLogxK

U2 - 10.1016/0021-9673(93)80479-R

DO - 10.1016/0021-9673(93)80479-R

M3 - Article

C2 - 8445006

AN - SCOPUS:0027476897

VL - 630

SP - 408

EP - 414

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -

Access to Document

10.1016/0021-9673(93)80479-R