Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis

Makoto Tsunoda, Masaru Kato, Takeshi Fukushima, Tomofumi Santa, Hiroshi Homma, Hiroko Yanai, Tomoyoshi Soga, Kazuhiro Imai

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalBiomedical Chromatography
Volume13
Issue number5
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Capillary electrophoresis
Enantiomers
Cyclodextrins
Capillary Electrophoresis
Aspartic Acid
D-Aspartic Acid
Pineal Gland
Viperidae
Limit of Detection
Rats
Buffers
Phosphates
Food

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis. / Tsunoda, Makoto; Kato, Masaru; Fukushima, Takeshi; Santa, Tomofumi; Homma, Hiroshi; Yanai, Hiroko; Soga, Tomoyoshi; Imai, Kazuhiro.

In: Biomedical Chromatography, Vol. 13, No. 5, 1999, p. 335-339.

Research output: Contribution to journalArticle

Tsunoda, Makoto ; Kato, Masaru ; Fukushima, Takeshi ; Santa, Tomofumi ; Homma, Hiroshi ; Yanai, Hiroko ; Soga, Tomoyoshi ; Imai, Kazuhiro. / Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis. In: Biomedical Chromatography. 1999 ; Vol. 13, No. 5. pp. 335-339.
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AU - Fukushima, Takeshi

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AU - Homma, Hiroshi

AU - Yanai, Hiroko

AU - Soga, Tomoyoshi

AU - Imai, Kazuhiro

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