Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis

Makoto Tsunoda; Masaru Kato; Takeshi Fukushima; Tomofumi Santa; Hiroshi Homma; Hiroko Yanai; Tomoyoshi Soga; Kazuhiro Imai

doi:10.1002/(SICI)1099-0801(199908)13:5<335::AID-BMC868>3.0.CO;2-4

Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis

Makoto Tsunoda, Masaru Kato, Takeshi Fukushima, Tomofumi Santa, Hiroshi Homma, Hiroko Yanai, Tomoyoshi Soga, Kazuhiro Imai

Research output: Contribution to journal › Article

27 Citations (Scopus)

Abstract

Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.

Original language	English
Pages (from-to)	335-339
Number of pages	5
Journal	Biomedical Chromatography
Volume	13
Issue number	5
DOIs	https://doi.org/10.1002/(SICI)1099-0801(199908)13:5<335::AID-BMC868>3.0.CO;2-4
Publication status	Published - 1999 Aug 23
Externally published	Yes

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ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

Cite this

Tsunoda, M., Kato, M., Fukushima, T., Santa, T., Homma, H., Yanai, H., Soga, T., & Imai, K. (1999). Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis. Biomedical Chromatography, 13(5), 335-339. https://doi.org/10.1002/(SICI)1099-0801(199908)13:5<335::AID-BMC868>3.0.CO;2-4

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abstract = "Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.",

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AU - Tsunoda, Makoto

AU - Kato, Masaru

AU - Fukushima, Takeshi

AU - Santa, Tomofumi

AU - Homma, Hiroshi

AU - Yanai, Hiroko

AU - Soga, Tomoyoshi

AU - Imai, Kazuhiro

PY - 1999/8/23

Y1 - 1999/8/23

N2 - Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.

AB - Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.

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10.1002/(SICI)1099-0801(199908)13:5<335::AID-BMC868>3.0.CO;2-4