Abstract
Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.
| Original language | English |
|---|---|
| Pages (from-to) | 335-339 |
| Number of pages | 5 |
| Journal | Biomedical Chromatography |
| Volume | 13 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Pharmacology
Cite this
Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis. / Tsunoda, Makoto; Kato, Masaru; Fukushima, Takeshi; Santa, Tomofumi; Homma, Hiroshi; Yanai, Hiroko; Soga, Tomoyoshi; Imai, Kazuhiro.
In: Biomedical Chromatography, Vol. 13, No. 5, 1999, p. 335-339.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of aspartic acid enantiomers in bio-samples by capillary electrophoresis
AU - Tsunoda, Makoto
AU - Kato, Masaru
AU - Fukushima, Takeshi
AU - Santa, Tomofumi
AU - Homma, Hiroshi
AU - Yanai, Hiroko
AU - Soga, Tomoyoshi
AU - Imai, Kazuhiro
PY - 1999
Y1 - 1999
N2 - Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.
AB - Enantiomeric separation and detection of D,L-aspartic acid (Asp) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) by capillary electrophoresis (CE) using modified cyclodextrins as chiral selectors was studied. Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin(TM-β-CD) was most effective for enantiomeric separation of NBD-D,L-Asp with optimum conditions of 30 mM TM-β-CD in 50 mM phosphate buffer (pH 4.0) and the limit of detection (LOD) attained was 100 mM for each enantiomer. The method proposed in the present study was convenient for both D- and L-Asp determination since the other amino compounds migrated differently and D-Asp in bio-samples such as rat pineal gland and foods was determined with a simple sample pretreatment and a short analysis run time.
UR - http://www.scopus.com/inward/record.url?scp=0032780479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032780479&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1099-0801(199908)13:5<335::AID-BMC868>3.0.CO;2-4
DO - 10.1002/(SICI)1099-0801(199908)13:5<335::AID-BMC868>3.0.CO;2-4
M3 - Article
C2 - 10425023
AN - SCOPUS:0032780479
VL - 13
SP - 335
EP - 339
JO - Biomedical Chromatography
JF - Biomedical Chromatography
SN - 0269-3879
IS - 5
ER -