Determination of carbohydrates by hydrophilic interaction chromatography with pulsed amperometric detection using postcolumn pH adjustment

Tomoyoshi Soga, Yoshinori Inoue, Kenji Yamaguchi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An HPLC method for the determination of carbohydrates using a postcolumn pH adjustment technique with lithium hydroxide solution followed by pulsed amperometric detection was developed. In conjunction with hydrophilic interaction chromatography, the technique was used to determine reducing sugars, non-reducing sugars and sugar alcohols. Detection limits from 0.7 to 2.7 ng with a signal-to-noise ratio of 3 were achieved. The relative standard deviation for peak was better than 2.7%. The postcolumn pH adjustment technique enabled gradient elution to be used.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalJournal of Chromatography A
Volume625
Issue number2
DOIs
Publication statusPublished - 1992 Nov 20
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Determination of carbohydrates by hydrophilic interaction chromatography with pulsed amperometric detection using postcolumn pH adjustment'. Together they form a unique fingerprint.

  • Cite this