Abstract
An automated method of analysis of catecholamines using a new packing material, internal surface boronic acid gel, was developed. The new support is designed with a carboxymethylcellulose-bonded external surface in order to be non-adsorptive to proteins and with a phenylboronic acid-bonded internal surface to retain only catecholamines. This packing support displayed an affinity for basic or neutral catecholic compounds with no protein adsorption and enabled on-line sample pretreatment of catecholamines in urine and deproteinized plasma. The catecholamines were selectively adsorbed on the new material and separated on a reversed-phase or a cation-exchange column. These compounds were then detected electrochemically. The limits of quantitation were 1.5-3.0 ng/ml in urine and 10-15 pg/ml in plasma, at a signal-to-noise ratio of 5.
| Original language | English |
|---|---|
| Pages (from-to) | 175-181 |
| Number of pages | 7 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 620 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1993 Oct 29 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Chemistry(all)
- Clinical Biochemistry
- Molecular Medicine
Cite this
Determination of catecholamines in urine and plasma by on-line sample pretreatment using an internal surface boronic acid gel. / Soga, Tomoyoshi; Inoue, Yoshinori.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 620, No. 2, 29.10.1993, p. 175-181.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of catecholamines in urine and plasma by on-line sample pretreatment using an internal surface boronic acid gel
AU - Soga, Tomoyoshi
AU - Inoue, Yoshinori
PY - 1993/10/29
Y1 - 1993/10/29
N2 - An automated method of analysis of catecholamines using a new packing material, internal surface boronic acid gel, was developed. The new support is designed with a carboxymethylcellulose-bonded external surface in order to be non-adsorptive to proteins and with a phenylboronic acid-bonded internal surface to retain only catecholamines. This packing support displayed an affinity for basic or neutral catecholic compounds with no protein adsorption and enabled on-line sample pretreatment of catecholamines in urine and deproteinized plasma. The catecholamines were selectively adsorbed on the new material and separated on a reversed-phase or a cation-exchange column. These compounds were then detected electrochemically. The limits of quantitation were 1.5-3.0 ng/ml in urine and 10-15 pg/ml in plasma, at a signal-to-noise ratio of 5.
AB - An automated method of analysis of catecholamines using a new packing material, internal surface boronic acid gel, was developed. The new support is designed with a carboxymethylcellulose-bonded external surface in order to be non-adsorptive to proteins and with a phenylboronic acid-bonded internal surface to retain only catecholamines. This packing support displayed an affinity for basic or neutral catecholic compounds with no protein adsorption and enabled on-line sample pretreatment of catecholamines in urine and deproteinized plasma. The catecholamines were selectively adsorbed on the new material and separated on a reversed-phase or a cation-exchange column. These compounds were then detected electrochemically. The limits of quantitation were 1.5-3.0 ng/ml in urine and 10-15 pg/ml in plasma, at a signal-to-noise ratio of 5.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027423738&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(93)80001-K
DO - 10.1016/0378-4347(93)80001-K
M3 - Article
VL - 620
SP - 175
EP - 181
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 0378-4347
IS - 2
ER -