Determination of cathepsin v activity and intracellular trafficking by N-glycosylation

Yuki Niwa, Takehiro Suzuki, Naoshi Dohmae, Kazuo Umezawa, Siro Simizu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Cathepsin V (L2), a lysosomal cysteine protease, is a member of cathepsin family, relating to cancer invasion and metastasis. Cathepsin V contains two predicted N-glycosylation sites, but it has not been reported whether cathepsin V is glycosylated or not. In this study, we clarified the role of N-glycosylation of cathepsin V for its functions. We demonstrated that cathepsin V is N-glycosylated at both Asn221 and Asn292 using mass spectrometry and site-directed mutagenesis. N-glycosylation of cathepsin V was important for transportation to lysosome, secretion, and activity in HT1080 cells. These data demonstrated that functions of cathepsin V are controlled by N-glycosylation.

Original languageEnglish
Pages (from-to)3601-3607
Number of pages7
JournalFEBS Letters
Volume586
Issue number20
DOIs
Publication statusPublished - 2012 Oct 19

Fingerprint

Glycosylation
Cathepsins
Cysteine Proteases
Site-Directed Mutagenesis
Lysosomes
Mutagenesis
Mass Spectrometry
Neoplasm Metastasis
Mass spectrometry
Neoplasms

Keywords

  • Cathepsin L2
  • Cathepsin V
  • Glycosylation
  • Invasion
  • Trafficking

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Determination of cathepsin v activity and intracellular trafficking by N-glycosylation. / Niwa, Yuki; Suzuki, Takehiro; Dohmae, Naoshi; Umezawa, Kazuo; Simizu, Siro.

In: FEBS Letters, Vol. 586, No. 20, 19.10.2012, p. 3601-3607.

Research output: Contribution to journalArticle

Niwa, Yuki ; Suzuki, Takehiro ; Dohmae, Naoshi ; Umezawa, Kazuo ; Simizu, Siro. / Determination of cathepsin v activity and intracellular trafficking by N-glycosylation. In: FEBS Letters. 2012 ; Vol. 586, No. 20. pp. 3601-3607.
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