Determination of copper(II) chlorophyllin by reversed-phase high-performance liquid chromatography

H. Inoue, H. Yamashita, K. Furuya, Y. Nonomura, N. Yoshioka, S. Lib

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44 Citations (Scopus)

Abstract

Copper(II) chlorophyllin, consisting of copper(II) pheophorbide a, copper(II) chlorin e6, copper(II) rhodin g7 and copper (II) chlorin e4, was prepared and separated by semi-preparative high-performance liquid chromatography (HPLC). The components of copper(II) chlorophyllin were determined on a reversed-phase Inertsil ODS-2 column using a mobile phase of methanol-water (97:3, v/v) containing 1% (v/v) of acetic acid. Linear calibration plots were obtained for copper(II) chlorophyllin in the concentration range of 0-30 μg cm-3 with photometric detection at 407 or 423 nm. The detection limits of copper(II) pheophorbide a, copper(II) chlorin e6, copper(II) rhodin g7 and copper(II) chlorin e4 were 3.5, 1.5, 3.3 and 1.4 ng cm-3 with relative standard deviations (n = 10) of 1.8, 1.6, 5.2 and 3.6%, respectively. The reversed-phase HPLC method proposed here was demonstrated to be useful for the determination of the components of sodium copper(II) chlorophyllin.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalJournal of Chromatography A
Volume679
Issue number1
DOIs
Publication statusPublished - 1994 Sep 9

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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