Determination of erythromycin, clarithromycin, roxithromycin, and azithromycin in plasma by high-performance liquid chromatography with amperometric detection

Chieko Taninaka, Hisakazu Ohtani, Erika Hanada, Hajime Kotaki, Hitoshi Sato, Tatsuji Iga

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105 Citations (Scopus)


In this study, a high-performance liquid chromatographic method was developed for the quantitative determination of erythromycin (EM), roxithromycin (RXM), and azithromycin (AZM) in rat plasma with amperometric detection under a standardized common condition using clarithromycin (CAM) as an internal standard. This method was also proved to be applicable for the determination of CAM by employing RXM as an internal standard. Each drug was extracted from 150 μl of plasma sample spiked with internal standard under an alkaline condition with tert.-butyl methyl ether. The detector cell potential for the oxidation of the drugs was set at +950 mV. The linearity of the calibration curves were preserved over the concentration ranges of 0.1-10 μg/ml for EM and RXM, and 0.03-3.0 μg/ml for CAM and AZM. Coefficients of variation and relative error were less than 9% and ±7%, respectively. The analytical method presented here was proved to be useful for the investigation of the pharmacokinetic characteristics of EM, CAM, RXM, and AZM in rats. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)405-411
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number2
Publication statusPublished - 2000 Feb 11



  • Azithromycin
  • Clarithromycin
  • Erythromycin
  • Roxithromycin

ASJC Scopus subject areas

  • Chemistry(all)

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