Determination of erythromycin, clarithromycin, roxithromycin, and azithromycin in plasma by high-performance liquid chromatography with amperometric detection

Chieko Taninaka, Hisakazu Ohtani, Erika Hanada, Hajime Kotaki, Hitoshi Sato, Tatsuji Iga

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

In this study, a high-performance liquid chromatographic method was developed for the quantitative determination of erythromycin (EM), roxithromycin (RXM), and azithromycin (AZM) in rat plasma with amperometric detection under a standardized common condition using clarithromycin (CAM) as an internal standard. This method was also proved to be applicable for the determination of CAM by employing RXM as an internal standard. Each drug was extracted from 150 μl of plasma sample spiked with internal standard under an alkaline condition with tert.-butyl methyl ether. The detector cell potential for the oxidation of the drugs was set at +950 mV. The linearity of the calibration curves were preserved over the concentration ranges of 0.1-10 μg/ml for EM and RXM, and 0.03-3.0 μg/ml for CAM and AZM. Coefficients of variation and relative error were less than 9% and ±7%, respectively. The analytical method presented here was proved to be useful for the investigation of the pharmacokinetic characteristics of EM, CAM, RXM, and AZM in rats. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)405-411
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume738
Issue number2
DOIs
Publication statusPublished - 2000 Feb 11
Externally publishedYes

Fingerprint

Roxithromycin
Azithromycin
Clarithromycin
High performance liquid chromatography
Erythromycin
Plasmas
Rats
Methyl Ethers
Pharmacokinetics
Pharmaceutical Preparations
Calibration
Detectors
Oxidation
Liquids

Keywords

  • Azithromycin
  • Clarithromycin
  • Erythromycin
  • Roxithromycin

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Determination of erythromycin, clarithromycin, roxithromycin, and azithromycin in plasma by high-performance liquid chromatography with amperometric detection. / Taninaka, Chieko; Ohtani, Hisakazu; Hanada, Erika; Kotaki, Hajime; Sato, Hitoshi; Iga, Tatsuji.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 738, No. 2, 11.02.2000, p. 405-411.

Research output: Contribution to journalArticle

@article{9d5737fcbfc2437e8cd5a368f381144a,
title = "Determination of erythromycin, clarithromycin, roxithromycin, and azithromycin in plasma by high-performance liquid chromatography with amperometric detection",
abstract = "In this study, a high-performance liquid chromatographic method was developed for the quantitative determination of erythromycin (EM), roxithromycin (RXM), and azithromycin (AZM) in rat plasma with amperometric detection under a standardized common condition using clarithromycin (CAM) as an internal standard. This method was also proved to be applicable for the determination of CAM by employing RXM as an internal standard. Each drug was extracted from 150 μl of plasma sample spiked with internal standard under an alkaline condition with tert.-butyl methyl ether. The detector cell potential for the oxidation of the drugs was set at +950 mV. The linearity of the calibration curves were preserved over the concentration ranges of 0.1-10 μg/ml for EM and RXM, and 0.03-3.0 μg/ml for CAM and AZM. Coefficients of variation and relative error were less than 9{\%} and ±7{\%}, respectively. The analytical method presented here was proved to be useful for the investigation of the pharmacokinetic characteristics of EM, CAM, RXM, and AZM in rats. (C) 2000 Elsevier Science B.V.",
keywords = "Azithromycin, Clarithromycin, Erythromycin, Roxithromycin",
author = "Chieko Taninaka and Hisakazu Ohtani and Erika Hanada and Hajime Kotaki and Hitoshi Sato and Tatsuji Iga",
year = "2000",
month = "2",
day = "11",
doi = "10.1016/S0378-4347(99)00512-5",
language = "English",
volume = "738",
pages = "405--411",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Determination of erythromycin, clarithromycin, roxithromycin, and azithromycin in plasma by high-performance liquid chromatography with amperometric detection

AU - Taninaka, Chieko

AU - Ohtani, Hisakazu

AU - Hanada, Erika

AU - Kotaki, Hajime

AU - Sato, Hitoshi

AU - Iga, Tatsuji

PY - 2000/2/11

Y1 - 2000/2/11

N2 - In this study, a high-performance liquid chromatographic method was developed for the quantitative determination of erythromycin (EM), roxithromycin (RXM), and azithromycin (AZM) in rat plasma with amperometric detection under a standardized common condition using clarithromycin (CAM) as an internal standard. This method was also proved to be applicable for the determination of CAM by employing RXM as an internal standard. Each drug was extracted from 150 μl of plasma sample spiked with internal standard under an alkaline condition with tert.-butyl methyl ether. The detector cell potential for the oxidation of the drugs was set at +950 mV. The linearity of the calibration curves were preserved over the concentration ranges of 0.1-10 μg/ml for EM and RXM, and 0.03-3.0 μg/ml for CAM and AZM. Coefficients of variation and relative error were less than 9% and ±7%, respectively. The analytical method presented here was proved to be useful for the investigation of the pharmacokinetic characteristics of EM, CAM, RXM, and AZM in rats. (C) 2000 Elsevier Science B.V.

AB - In this study, a high-performance liquid chromatographic method was developed for the quantitative determination of erythromycin (EM), roxithromycin (RXM), and azithromycin (AZM) in rat plasma with amperometric detection under a standardized common condition using clarithromycin (CAM) as an internal standard. This method was also proved to be applicable for the determination of CAM by employing RXM as an internal standard. Each drug was extracted from 150 μl of plasma sample spiked with internal standard under an alkaline condition with tert.-butyl methyl ether. The detector cell potential for the oxidation of the drugs was set at +950 mV. The linearity of the calibration curves were preserved over the concentration ranges of 0.1-10 μg/ml for EM and RXM, and 0.03-3.0 μg/ml for CAM and AZM. Coefficients of variation and relative error were less than 9% and ±7%, respectively. The analytical method presented here was proved to be useful for the investigation of the pharmacokinetic characteristics of EM, CAM, RXM, and AZM in rats. (C) 2000 Elsevier Science B.V.

KW - Azithromycin

KW - Clarithromycin

KW - Erythromycin

KW - Roxithromycin

UR - http://www.scopus.com/inward/record.url?scp=0033962690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033962690&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(99)00512-5

DO - 10.1016/S0378-4347(99)00512-5

M3 - Article

C2 - 10718658

AN - SCOPUS:0033962690

VL - 738

SP - 405

EP - 411

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 0378-4347

IS - 2

ER -