Determination of erythromycin concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection

Erika Hanada, Hisakazu Ohtani, Hajime Kotaki, Yasufumi Sawada, Tatsuji Iga

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Abstract

A simple method for the quantitative determination of erythromycin (EM) concentrations in rat plasma and liver by high-performance liquid chromatography with amperometric detection was developed. EM was extracted from 200 μl of plasma or liver homogenate sample under sodium hydroxide alkaline conditions with tert.-butyl methyl ether. Oleandomycin was used as an internal standard. The recovery rate of EM was up to 100%. The detector cell potential for the oxidation of EM was +1100 mV. The calibration curves were linear over the concentration ranges 0.1-20.0 μg/ml for plasma and 0.5-100.0 μg/g for liver. The method was applied to the determination of the plasma and liver concentrations of EM in rats after intravenous administration (50 mg/kg dose). The method presented here has proved to be of great use for the investigation of the pharmacokinetic characteristics of EM in small animals such as rats.

Original languageEnglish
Pages (from-to)478-482
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Volume692
Issue number2
DOIs
Publication statusPublished - 1997 May 9
Externally publishedYes

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Keywords

  • Erythromycin

ASJC Scopus subject areas

  • Chemistry(all)

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