Determination of landiolol in human plasma by high-performance liquid chromatography with fluorescence detection

Tohru Aomori, Naoki Matsumoto, Katsunori Nakamura, Yukiyoshi Fujita, Masayuki Sekizuka, Takuya Araki, Tomonori Nakamura, Koujirou Yamamoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Landiolol is an ultra-short-acting β 1-adrenergic receptor-blocking agent that is used with perioperative patients who are at risk of cardiac events. The drug is hydrolyzed very rapidly by pseudocholinesterase in the blood. Using physostigmine (final concentration: 10 -4 mol/L) as a cholinesterase inhibitor, we developed a simple and rapid quantification method for landiolol using high-performance liquid chromatography. Chromatographic separations were achieved on a C 18 column with 20 mMKH 2PO 4/acetonitrile (70/30, v/v). Propranolol was used as an internal standard. The peaks were detected using a fluorescence detector (excitation 220 nm and emission 310 nm). The retention times of landiolol and propranolol were 5.6 min and 6.7 min, respectively. The lower limit of quantification was estimated as being 0.1 μg/mL. The precision of the inter-and intra-assay varied between 1.8% and 12.6%, and the accuracy varied between-8.1% and 7.7%. This method was useful for the determination of landiolol in human plasma after intravenous administration.

Original languageEnglish
Pages (from-to)2411-2419
Number of pages9
JournalJournal of Liquid Chromatography and Related Technologies
Volume34
Issue number19
DOIs
Publication statusPublished - 2011 Dec 1
Externally publishedYes

Keywords

  • HPLC-fluorescence detector
  • Landiolol
  • Physostigmine
  • Propanolol
  • Pseudocholinesterase
  • Rapid quantification method

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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