Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis

Hideyuki Nishizawa, Natsumi Kita, Sakiko Okimura, Emi Takao, Yoshihiro Abe

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm × 9 cm × 0.05 mm 4–20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.

Original languageEnglish
Pages (from-to)803-806
Number of pages4
JournalELECTROPHORESIS
Volume9
Issue number12
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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