Determination of omeprazole and its metabolites in human plasma by liquid chromatography-mass spectrometry

Hideko Kanazawa, Akiko Okada, Yoshikazu Matsushima, Hiromitsu Yokota, Shigeo Okubo, Fumiko Mashige, Kazuhiko Nakahara

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Omeprazole is a benzimidazole compound that acts as a proton-pump inhibitor. Because the metabolism of omeprazole is mainly catalyzed by cytochrome P-450 (CYP) 3A4 and CYP2C19, the genetic polymorphism of CYP2C19 could be of clinical concern in the treatment of acid-related diseases with omeprazole. Therefore, a reliable method for omeprazole phenotyping is desirable in clinical situations. This study has demonstrated the determination of omeprazole and its metabolites in human plasma by liquid chromatography-three-dimensional quadrupole mass spectrometry with a sonic spray ionization interface. The analytical column was YMC-Pack Pro C18(50×2.0 mm I.D.) using acetonitrile-50 mM ammonium acetate (pH 7.25) (1:4) at a flow-rate of 0.2 ml/min. The drift voltage was 30 V. The sampling aperture was heated at 110°C and Shield temperature was 230°C. In the mass spectrum, the molecular ions of omeprazole, hydroxyomeprazole and omeprazole sulfone were clearly observed as base peaks. This method is sufficiently sensitive and accurate for pharmacokinetic studies of omeprazol.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalJournal of Chromatography A
Volume949
Issue number1-2
DOIs
Publication statusPublished - 2002 Mar 8

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Plasma (human)
Omeprazole
Liquid chromatography
Metabolites
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Pharmacokinetics
Proton Pump Inhibitors
Genetic Polymorphisms
Polymorphism
Metabolism
Cytochrome P-450 Enzyme System
Ionization
Flow rate
Ions
Sampling
Temperature
Acids
Electric potential

Keywords

  • Benzimidazoles
  • Cytochromes
  • Interfaces, LC-MS
  • Omeprazole
  • Sonic spray ionization

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of omeprazole and its metabolites in human plasma by liquid chromatography-mass spectrometry. / Kanazawa, Hideko; Okada, Akiko; Matsushima, Yoshikazu; Yokota, Hiromitsu; Okubo, Shigeo; Mashige, Fumiko; Nakahara, Kazuhiko.

In: Journal of Chromatography A, Vol. 949, No. 1-2, 08.03.2002, p. 1-9.

Research output: Contribution to journalArticle

Kanazawa, Hideko ; Okada, Akiko ; Matsushima, Yoshikazu ; Yokota, Hiromitsu ; Okubo, Shigeo ; Mashige, Fumiko ; Nakahara, Kazuhiko. / Determination of omeprazole and its metabolites in human plasma by liquid chromatography-mass spectrometry. In: Journal of Chromatography A. 2002 ; Vol. 949, No. 1-2. pp. 1-9.
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