Determination of poor prognostic immune features of tumour microenvironment in non-smoking patients with lung adenocarcinoma

Tomonari Kinoshita, Chie Kudo-Saito, Reiko Muramatsu, Tomonobu Fujita, Miyuki Saito, Haruna Nagumo, Toshiharu Sakurai, Shinobu Noji, Emi Takahata, Tomonori Yaguchi, Nobuo Tsukamoto, Yuichiro Hayashi, Kaoru Kaseda, Ikuo Kamiyama, Takashi Ohtsuka, Kenji Tomizawa, Masaki Shimoji, Tetsuya Mitsudomi, Hisao Asamura, Yutaka Kawakami

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have previously demonstrated that the prognostic significance of tumour-infiltrating CD8+ T cells significantly differs according to histological type and patient smoking habits in non-small cell lung cancer (NSCLC). This work suggested that infiltrating CD8+ T cells may not be activated sufficiently in the immunosuppressive microenvironment in non-smokers with adenocarcinoma. To understand the immunogenic microenvironment in NSCLC, we characterised immune cells comprehensively by performing an immunohistochemical evaluation using an alternative counting method and multicolour staining method (n = 234), and assessed immune-related gene expression by using genetic analytical approaches (n = 58). We found that high infiltration of activated CD8+ T cells expressing interferon gamma (IFN-γ) and granzyme was correlated with postoperative survival in patients with non-adenocarcinoma. On the contrary, CD8+ T-cell accumulation was identified as a worse prognostic factor in patients with adenocarcinoma, particularly in non-smokers. Infiltrating CD8+ T cells were significantly less activated in this microenvironment with high expression of various immunoregulation genes. Potentially immunoregulatory CD8+ FOXP3+ T cells and immunodysfunctional CD8+ GATA3+ T cells were increased in adenocarcinoma of non-smokers. CD4+ FOXP3+ regulatory T cells expressing chemokine receptor-4 (CCR4)- and chemokine ligand (CCL17)-expressing CD163+ M2-like macrophages also accumulated correlatively and significantly in adenocarcinoma of non-smokers. These characteristic immune cells may promote tumour progression possibly by creating an immunosuppressive microenvironment in non-smoking patients with lung adenocarcinoma. Our findings may be helpful for refining the current strategy of personalised immunotherapy including immune-checkpoint blockade therapy for NSCLC.

Original languageEnglish
Pages (from-to)15-27
Number of pages13
JournalEuropean Journal of Cancer
Volume86
DOIs
Publication statusPublished - 2017 Nov 1

Fingerprint

Tumor Microenvironment
T-Lymphocytes
Adenocarcinoma
Non-Small Cell Lung Carcinoma
Immunosuppressive Agents
Chemokine CCL17
Granzymes
Chemokine Receptors
Regulatory T-Lymphocytes
Adenocarcinoma of lung
Immunotherapy
Interferon-gamma
Habits
Neoplasms
Smoking
Macrophages
Staining and Labeling
Ligands
Gene Expression
Survival

Keywords

  • Adenocarcinoma
  • Immunosuppression
  • Non-small cell lung cancer
  • Smoking history
  • Tumour microenvironment

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Determination of poor prognostic immune features of tumour microenvironment in non-smoking patients with lung adenocarcinoma. / Kinoshita, Tomonari; Kudo-Saito, Chie; Muramatsu, Reiko; Fujita, Tomonobu; Saito, Miyuki; Nagumo, Haruna; Sakurai, Toshiharu; Noji, Shinobu; Takahata, Emi; Yaguchi, Tomonori; Tsukamoto, Nobuo; Hayashi, Yuichiro; Kaseda, Kaoru; Kamiyama, Ikuo; Ohtsuka, Takashi; Tomizawa, Kenji; Shimoji, Masaki; Mitsudomi, Tetsuya; Asamura, Hisao; Kawakami, Yutaka.

In: European Journal of Cancer, Vol. 86, 01.11.2017, p. 15-27.

Research output: Contribution to journalArticle

Kinoshita, T, Kudo-Saito, C, Muramatsu, R, Fujita, T, Saito, M, Nagumo, H, Sakurai, T, Noji, S, Takahata, E, Yaguchi, T, Tsukamoto, N, Hayashi, Y, Kaseda, K, Kamiyama, I, Ohtsuka, T, Tomizawa, K, Shimoji, M, Mitsudomi, T, Asamura, H & Kawakami, Y 2017, 'Determination of poor prognostic immune features of tumour microenvironment in non-smoking patients with lung adenocarcinoma', European Journal of Cancer, vol. 86, pp. 15-27. https://doi.org/10.1016/j.ejca.2017.08.026
Kinoshita, Tomonari ; Kudo-Saito, Chie ; Muramatsu, Reiko ; Fujita, Tomonobu ; Saito, Miyuki ; Nagumo, Haruna ; Sakurai, Toshiharu ; Noji, Shinobu ; Takahata, Emi ; Yaguchi, Tomonori ; Tsukamoto, Nobuo ; Hayashi, Yuichiro ; Kaseda, Kaoru ; Kamiyama, Ikuo ; Ohtsuka, Takashi ; Tomizawa, Kenji ; Shimoji, Masaki ; Mitsudomi, Tetsuya ; Asamura, Hisao ; Kawakami, Yutaka. / Determination of poor prognostic immune features of tumour microenvironment in non-smoking patients with lung adenocarcinoma. In: European Journal of Cancer. 2017 ; Vol. 86. pp. 15-27.
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AU - Saito, Miyuki

AU - Nagumo, Haruna

AU - Sakurai, Toshiharu

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AU - Tsukamoto, Nobuo

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AU - Tomizawa, Kenji

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AU - Mitsudomi, Tetsuya

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