Determination of sedatives and anesthetics in plasma by liquid chromatography-mass spectrometry with a desalting system

Hideko Kanazawa, Yoshiko Konishi, Yoshikazu Matsushima, Toshiaki Takahashi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Though liquid-chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites, it does not allow the use of a non-volatile buffer such as phosphate buffer. We used a column-switching de, salting system in combination with atmospheric pressure chemical ionization LC-MS for analysis of sedatives and anaesthetics. The drugs examined were flumazenil, butorphanol, midazolam, lorazepam, phenobarbital and flunitrazepam. The separation was carried out on a reversed-phase column using acetonitrile-0.1 M phosphate buffer as a mobile phase. The mass spectra are almost the same as those obtained by direct analysis and the molecular ions were clearly observed. In the analysis, phosphate buffer was completely removed with the trapping column and did not interfere with ionization of the drugs in MS. The chiral separation of lorazepam was achieved on a chiral column with UV, optical rotatory detection and MS. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs in biological samples.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalJournal of Chromatography A
Volume797
Issue number1-2
DOIs
Publication statusPublished - 1998 Feb 27

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Salt removal
Liquid chromatography
Hypnotics and Sedatives
Liquid Chromatography
Mass spectrometry
Anesthetics
Mass Spectrometry
Buffers
Plasmas
Lorazepam
Phosphates
Pharmaceutical Preparations
Ionization
Butorphanol
Flunitrazepam
Flumazenil
Atmospheric Pressure
Pharmacokinetics
Midazolam
Phenobarbital

Keywords

  • Anaesthetics
  • Desalting methods
  • Enantiomer separation
  • Sedatives

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of sedatives and anesthetics in plasma by liquid chromatography-mass spectrometry with a desalting system. / Kanazawa, Hideko; Konishi, Yoshiko; Matsushima, Yoshikazu; Takahashi, Toshiaki.

In: Journal of Chromatography A, Vol. 797, No. 1-2, 27.02.1998, p. 227-236.

Research output: Contribution to journalArticle

Kanazawa, Hideko ; Konishi, Yoshiko ; Matsushima, Yoshikazu ; Takahashi, Toshiaki. / Determination of sedatives and anesthetics in plasma by liquid chromatography-mass spectrometry with a desalting system. In: Journal of Chromatography A. 1998 ; Vol. 797, No. 1-2. pp. 227-236.
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