Determination of theophylline and its metabolites in biological samples by liquid chromatography-mass spectrometry

Hideko Kanazawa, Ryoko Atsumi, Yoshikazu Matsushima, Junko Kizu

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites. We used a column-switching system in combination with atmospheric pressure chemical ionization LC-MS (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. The separation was carried out on a reversed-phase column using methanol-20 mM ammonium acetate as a mobile phase at a flow-rate of 1 ml/min in 30 min. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)87-96
Number of pages10
JournalJournal of Chromatography A
Volume870
Issue number1-2
DOIs
Publication statusPublished - 2000 Feb 18

Fingerprint

Liquid chromatography
Theophylline
Metabolites
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Pharmaceutical Preparations
Atmospheric Pressure
Pharmacokinetics
Switching systems
Atmospheric pressure
Ionization
Methanol
Flow rate
Ions

Keywords

  • Caffeine
  • Column switching
  • Theophylline

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of theophylline and its metabolites in biological samples by liquid chromatography-mass spectrometry. / Kanazawa, Hideko; Atsumi, Ryoko; Matsushima, Yoshikazu; Kizu, Junko.

In: Journal of Chromatography A, Vol. 870, No. 1-2, 18.02.2000, p. 87-96.

Research output: Contribution to journalArticle

@article{b364b2e9dbdb48f69344282ec1732fba,
title = "Determination of theophylline and its metabolites in biological samples by liquid chromatography-mass spectrometry",
abstract = "Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites. We used a column-switching system in combination with atmospheric pressure chemical ionization LC-MS (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. The separation was carried out on a reversed-phase column using methanol-20 mM ammonium acetate as a mobile phase at a flow-rate of 1 ml/min in 30 min. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Caffeine, Column switching, Theophylline",
author = "Hideko Kanazawa and Ryoko Atsumi and Yoshikazu Matsushima and Junko Kizu",
year = "2000",
month = "2",
day = "18",
doi = "10.1016/S0021-9673(99)00891-2",
language = "English",
volume = "870",
pages = "87--96",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Determination of theophylline and its metabolites in biological samples by liquid chromatography-mass spectrometry

AU - Kanazawa, Hideko

AU - Atsumi, Ryoko

AU - Matsushima, Yoshikazu

AU - Kizu, Junko

PY - 2000/2/18

Y1 - 2000/2/18

N2 - Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites. We used a column-switching system in combination with atmospheric pressure chemical ionization LC-MS (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. The separation was carried out on a reversed-phase column using methanol-20 mM ammonium acetate as a mobile phase at a flow-rate of 1 ml/min in 30 min. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs. Copyright (C) 2000 Elsevier Science B.V.

AB - Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for analysis of drugs and their metabolites. We used a column-switching system in combination with atmospheric pressure chemical ionization LC-MS (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. The separation was carried out on a reversed-phase column using methanol-20 mM ammonium acetate as a mobile phase at a flow-rate of 1 ml/min in 30 min. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. This method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs. Copyright (C) 2000 Elsevier Science B.V.

KW - Caffeine

KW - Column switching

KW - Theophylline

UR - http://www.scopus.com/inward/record.url?scp=0033956667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033956667&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(99)00891-2

DO - 10.1016/S0021-9673(99)00891-2

M3 - Article

VL - 870

SP - 87

EP - 96

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1-2

ER -