Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides

Noboru Fujimoto, Hideaki Tokai, Kazushi Iwata, Yasunori Okada, Taro Hayakawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalJournal of Immunological Methods
Volume187
Issue number1
Publication statusPublished - 1995 Nov 16

Fingerprint

Tissue Inhibitor of Metalloproteinase-2
Oligopeptides
Monoclonal Antibodies
Immunoenzyme Techniques
Guinea Pigs
Rabbits
Serum
Antibodies

Keywords

  • Enzyme immunoassay
  • Matrix metalloproteinase
  • Monoclonal antibody
  • Tissue inhibitor of metalloproteinases-2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biotechnology

Cite this

Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides. / Fujimoto, Noboru; Tokai, Hideaki; Iwata, Kazushi; Okada, Yasunori; Hayakawa, Taro.

In: Journal of Immunological Methods, Vol. 187, No. 1, 16.11.1995, p. 33-39.

Research output: Contribution to journalArticle

Fujimoto, Noboru ; Tokai, Hideaki ; Iwata, Kazushi ; Okada, Yasunori ; Hayakawa, Taro. / Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides. In: Journal of Immunological Methods. 1995 ; Vol. 187, No. 1. pp. 33-39.
@article{76000668ed5240f9b11a97b0a86bb762,
title = "Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides",
abstract = "We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.",
keywords = "Enzyme immunoassay, Matrix metalloproteinase, Monoclonal antibody, Tissue inhibitor of metalloproteinases-2",
author = "Noboru Fujimoto and Hideaki Tokai and Kazushi Iwata and Yasunori Okada and Taro Hayakawa",
year = "1995",
month = "11",
day = "16",
language = "English",
volume = "187",
pages = "33--39",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides

AU - Fujimoto, Noboru

AU - Tokai, Hideaki

AU - Iwata, Kazushi

AU - Okada, Yasunori

AU - Hayakawa, Taro

PY - 1995/11/16

Y1 - 1995/11/16

N2 - We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.

AB - We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.

KW - Enzyme immunoassay

KW - Matrix metalloproteinase

KW - Monoclonal antibody

KW - Tissue inhibitor of metalloproteinases-2

UR - http://www.scopus.com/inward/record.url?scp=0028800424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028800424&partnerID=8YFLogxK

M3 - Article

VL - 187

SP - 33

EP - 39

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -