Determination of topological structure of ARL6ip1 in cells: Identification of the essential binding region of ARL6ip1 for conophylline

Masahiro Kuroda; Shintaro Funasaki; Tsuyoshi Saitoh; Yukiko Sasazawa; Shigeru Nishiyama; Kazuo Umezawa; Siro Simizu

doi:10.1016/j.febslet.2013.09.017

Determination of topological structure of ARL6ip1 in cells: Identification of the essential binding region of ARL6ip1 for conophylline

Masahiro Kuroda, Shintaro Funasaki, Tsuyoshi Saitoh, Yukiko Sasazawa, Shigeru Nishiyama, Kazuo Umezawa, Siro Simizu

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.

Original language	English
Pages (from-to)	3656-3660
Number of pages	5
Journal	FEBS Letters
Volume	587
Issue number	22
DOIs	https://doi.org/10.1016/j.febslet.2013.09.017
Publication status	Published - 2013 Nov 15

Keywords

ARL6ip1
Conophylline
Redox-sensitive luciferase assay
Topology

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2013.09.017

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title = "Determination of topological structure of ARL6ip1 in cells: Identification of the essential binding region of ARL6ip1 for conophylline",

abstract = "Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.",

keywords = "ARL6ip1, Conophylline, Redox-sensitive luciferase assay, Topology",

author = "Masahiro Kuroda and Shintaro Funasaki and Tsuyoshi Saitoh and Yukiko Sasazawa and Shigeru Nishiyama and Kazuo Umezawa and Siro Simizu",

note = "Funding Information: This work was supported financially in part by Grant-in-Aid for Scientific Research (B) ( 23310163 and 24310167 ) and a Grant-in-Aid for Young Scientists (Start-up: 24810025 ) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan , and the Nateglinide Memorial Toyoshima Research and Education Fund of Keio University . ",

year = "2013",

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doi = "10.1016/j.febslet.2013.09.017",

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AU - Kuroda, Masahiro

AU - Funasaki, Shintaro

AU - Saitoh, Tsuyoshi

AU - Sasazawa, Yukiko

AU - Nishiyama, Shigeru

AU - Umezawa, Kazuo

AU - Simizu, Siro

N1 - Funding Information: This work was supported financially in part by Grant-in-Aid for Scientific Research (B) ( 23310163 and 24310167 ) and a Grant-in-Aid for Young Scientists (Start-up: 24810025 ) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan , and the Nateglinide Memorial Toyoshima Research and Education Fund of Keio University .

PY - 2013/11/15

Y1 - 2013/11/15

N2 - Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.

AB - Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.

KW - ARL6ip1

KW - Conophylline

KW - Redox-sensitive luciferase assay

KW - Topology

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Determination of topological structure of ARL6ip1 in cells: Identification of the essential binding region of ARL6ip1 for conophylline

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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