Determination of topological structure of ARL6ip1 in cells: Identification of the essential binding region of ARL6ip1 for conophylline

Masahiro Kuroda, Shintaro Funasaki, Tsuyoshi Saitoh, Yukiko Sasazawa, Shigeru Nishiyama, Kazuo Umezawa, Siro Simizu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.

Original languageEnglish
Pages (from-to)3656-3660
Number of pages5
JournalFEBS Letters
Volume587
Issue number22
DOIs
Publication statusPublished - 2013 Nov 15

Fingerprint

ADP-Ribosylation Factors
Proteins
conophylline
ADP-ribosylation factor 6
Sequence Deletion
Bioactivity
Insulin

Keywords

  • ARL6ip1
  • Conophylline
  • Redox-sensitive luciferase assay
  • Topology

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Determination of topological structure of ARL6ip1 in cells : Identification of the essential binding region of ARL6ip1 for conophylline. / Kuroda, Masahiro; Funasaki, Shintaro; Saitoh, Tsuyoshi; Sasazawa, Yukiko; Nishiyama, Shigeru; Umezawa, Kazuo; Simizu, Siro.

In: FEBS Letters, Vol. 587, No. 22, 15.11.2013, p. 3656-3660.

Research output: Contribution to journalArticle

Kuroda, Masahiro ; Funasaki, Shintaro ; Saitoh, Tsuyoshi ; Sasazawa, Yukiko ; Nishiyama, Shigeru ; Umezawa, Kazuo ; Simizu, Siro. / Determination of topological structure of ARL6ip1 in cells : Identification of the essential binding region of ARL6ip1 for conophylline. In: FEBS Letters. 2013 ; Vol. 587, No. 22. pp. 3656-3660.
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