Development and application of high-performance affinity beads: Toward chemical biology and drug discovery

Satoshi Sakamoto, Yasuaki Kabe, Mamoru Hatakeyama, Yuki Yamaguchi, Hiroshi Handa

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

In drug development research, the elucidation and understanding of the interactions between physiologically active substances and proteins that numerous genes produce is important. Currently, most commercially available drugs and physiologically active substances have been brought to market without knowledge of factors interacting with the drugs and the substances. Affinity purification is a useful and powerful technique employed to understand factors that are targeted by drugs and physiologically active substances. However, use of conventional matrices for affinity chromatography often causes a decrease in efficiency of affinity purification and, as a result, more practical matrices for affinity purification have been developed for application in drug discovery research. In this paper, we describe the development of high-performance affinity beads (SG beads and FG beads) that enable one-step affinity purification of drug targets and the elucidation of the mechanism of the action of the drugs. We also describe a chemical screening system using our affinity beads. We hope that utilization of the affinity beads will contribute to the progress of research in chemical biology.

Original languageEnglish
Pages (from-to)66-85
Number of pages20
JournalChemical Record
Volume9
Issue number1
DOIs
Publication statusPublished - 2009 Mar 5

Keywords

  • Affinity purification
  • Chemical screening system
  • Drug discovery research
  • Latex beads
  • Magnetic beads

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Chemical Engineering(all)
  • Materials Chemistry

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