Development and partial characterisation of an antiserum against apolipoprotein B of the short-finned eel, Anguilla australis

Erin L. Damsteegt, Hiroko Mizuta, Yuichi Ozaki, Naoshi Hiramatsu, Takashi Todo, Akihiko Hara, Shigeho Ijiri, Shinji Adachi, P. Mark Lokman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Despite its key role in transportation of triacylglycerides in blood, the distribution, localisation and molecular weight variants of apolipoprotein B (Apob) in teleost fish have essentially escaped study. To address this, a specific short-finned eel (Anguilla australis) Apob antiserum was produced by an immunised rabbit, purified and partially characterised. Localisation of Apob at both the mRNA (in situ hybridisation) and protein (immunohistochemistry) levels mirrored that of mammals; thus immunostaining was confined to the interstitial spaces of the liver and the vascular core of the intestinal villi. Immunostaining of proteins by Western blotting, followed by high-resolution LC-MS, indicated that peptide sequence coverage of Apob in low-density lipoproteins spanned the full-length protein. We conclude that only full-length Apob is produced by eels and that both liver and intestine are key sites for its synthesis.

Original languageEnglish
Pages (from-to)589-599
Number of pages11
JournalJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Volume184
Issue number5
DOIs
Publication statusPublished - 2014 Jun

Keywords

  • Anguilla australis
  • Antiserum
  • Apolipoprotein B
  • Immunohistochemistry
  • In situ hybridisation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Physiology
  • Animal Science and Zoology
  • Endocrinology

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