Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases

Shota Shimokihara, Shunya Shitara, Ryo Oyobiki, Takeshi Watanabe, Yasuaki Einaga, Yoshinori Matsumoto, Kei Fujiwara, Kenichi Horisawa, Nobuhide Doi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1302-1304
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

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Keywords

  • Biosensing
  • Directed evolution
  • High-throughput screening
  • Oxidoreductase

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Matsumoto, Y., Fujiwara, K., Horisawa, K., & Doi, N. (2015). Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1302-1304). Chemical and Biological Microsystems Society.