TY - GEN
T1 - Development of 105 plate number liquid choromatography using extended-nanofluidic channel
AU - Morikawa, Kyojiro
AU - Kazoe, Yutaka
AU - Shimizu, Hisashi
AU - Shimizu, Yusuke
AU - Mawatari, Kazuma
AU - Kitamori, Takehiko
N1 - Funding Information:
The authors acknowledge Prof. Shigeru Terabe at University of Hyogo for fruitful discussion. This work was supported by a Social Cooperation Program on Next-Generation Analytical Instrumentation in School of Engineering, The University of Tokyo, Japan.
Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
PY - 2018
Y1 - 2018
N2 - We developed pressure driven reverse phase liquid chromatography using an extended-nano channel (10-1000 nm) for 105 plate numbers. The chromatography was performed using a 100 mm long extended-nano channel (the world's longest extended-nano channel) with 5 pL injection volume. The obtained theoretical plate numbers 7.2-8.9×104 were well accorded with theoretically-estimated one. From these results, performance of the system was verified, and it has potential to realize 105 plates for protein and peptide separation. This technique will be a strong tool for analysis of ultrasmall biological samples like a single cell.
AB - We developed pressure driven reverse phase liquid chromatography using an extended-nano channel (10-1000 nm) for 105 plate numbers. The chromatography was performed using a 100 mm long extended-nano channel (the world's longest extended-nano channel) with 5 pL injection volume. The obtained theoretical plate numbers 7.2-8.9×104 were well accorded with theoretically-estimated one. From these results, performance of the system was verified, and it has potential to realize 105 plates for protein and peptide separation. This technique will be a strong tool for analysis of ultrasmall biological samples like a single cell.
KW - Extended-nano space
KW - Liquid chromatography
KW - Nanofluidics
KW - Pressure-driven flow
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M3 - Conference contribution
AN - SCOPUS:85061537213
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 2038
EP - 2039
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -