Development of 105 plate number liquid choromatography using extended-nanofluidic channel

Kyojiro Morikawa, Yutaka Kazoe, Hisashi Shimizu, Yusuke Shimizu, Kazuma Mawatari, Takehiko Kitamori

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We developed pressure driven reverse phase liquid chromatography using an extended-nano channel (10-1000 nm) for 105 plate numbers. The chromatography was performed using a 100 mm long extended-nano channel (the world's longest extended-nano channel) with 5 pL injection volume. The obtained theoretical plate numbers 7.2-8.9×104 were well accorded with theoretically-estimated one. From these results, performance of the system was verified, and it has potential to realize 105 plates for protein and peptide separation. This technique will be a strong tool for analysis of ultrasmall biological samples like a single cell.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages2038-2039
Number of pages2
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Externally publishedYes
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume4

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period18/11/1118/11/15

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Keywords

  • Extended-nano space
  • Liquid chromatography
  • Nanofluidics
  • Pressure-driven flow

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

Cite this

Morikawa, K., Kazoe, Y., Shimizu, H., Shimizu, Y., Mawatari, K., & Kitamori, T. (2018). Development of 105 plate number liquid choromatography using extended-nanofluidic channel. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 2038-2039). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 4). Chemical and Biological Microsystems Society.