Development of a far-red to near-infrared fluorescence probe for calcium ion and its application to multicolor neuronal imaging

Takahiro Egawa, Kenjiro Hanaoka, Yuichiro Koide, Sakiko Ujita, Naoya Takahashi, Yuji Ikegaya, Norio Matsuki, Takuya Terai, Tasuku Ueno, Toru Komatsu, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

154 Citations (Scopus)

Abstract

To improve optical imaging of Ca 2+ and to make available a distinct color window for multicolor imaging, we designed and synthesized CaSiR-1, a far-red to near-infrared fluorescence probe for Ca 2+, using Si-rhodamine (SiR) as the fluorophore and the well-known Ca 2+ chelator BAPTA. This wavelength region is advantageous, affording higher tissue penetration, lower background autofluorescence, and lower phototoxicity in comparison with the UV to visible range. CaSiR-1 has a high fluorescence off/on ratio of over 1000. We demonstrate its usefulness for multicolor fluorescence imaging of action potentials (visualized as increases in intracellular Ca 2+) in brain slices loaded with sulforhodamine 101 (red color; specific for astrocytes) that were prepared from transgenic mice in which some neurons expressed green fluorescent protein.

Original languageEnglish
Pages (from-to)14157-14159
Number of pages3
JournalJournal of the American Chemical Society
Volume133
Issue number36
DOIs
Publication statusPublished - 2011 Sep 14
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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