Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region

Takahiro Egawa; Yuichiro Koide; Kenjiro Hanaoka; Toru Komatsu; Takuya CooTeraiper; Tetsuo Nagano

doi:10.1039/c1cc00078k

Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region

Takahiro Egawa, Yuichiro Koide, Kenjiro Hanaoka, Toru Komatsu, Takuya CooTeraiper, Tetsuo Nagano

Research output: Contribution to journal › Article › peer-review

136 Citations (Scopus)

Abstract

We present a design strategy for fluorescence probes with a high off/on activation ratio in the red wavelength region, based on a novel fluorescein analogue in which the O atom at the 10 position of the xanthene chromophore is replaced with a Si atom. To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for β-galactosidase, and showed that it works in live HEK293 cells.

Original language	English
Pages (from-to)	4162-4164
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	14
DOIs	https://doi.org/10.1039/c1cc00078k
Publication status	Published - 2011 Mar 22
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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AU - Egawa, Takahiro

AU - Koide, Yuichiro

AU - Hanaoka, Kenjiro

AU - Komatsu, Toru

AU - CooTeraiper, Takuya

AU - Nagano, Tetsuo

PY - 2011/3/22

Y1 - 2011/3/22

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AB - We present a design strategy for fluorescence probes with a high off/on activation ratio in the red wavelength region, based on a novel fluorescein analogue in which the O atom at the 10 position of the xanthene chromophore is replaced with a Si atom. To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for β-galactosidase, and showed that it works in live HEK293 cells.

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Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region

Abstract

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