Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities

Yugo Kuriki; Takafusa Yoshioka; Mako Kamiya; Toru Komatsu; Hiroyuki Takamaru; Kyohhei Fujita; Hirohisa Iwaki; Aika Nanjo; Yuki Akagi; Kohei Takeshita; Haruaki Hino; Rumi Hino; Ryosuke Kojima; Tasuku Ueno; Kenjiro Hanaoka; Seiichiro Abe; Yutaka Saito; Jun Nakajima; Yasuteru Urano

doi:10.1039/d1sc06889j

Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities

Yugo Kuriki, Takafusa Yoshioka, Mako Kamiya, Toru Komatsu, Hiroyuki Takamaru, Kyohhei Fujita, Hirohisa Iwaki, Aika Nanjo, Yuki Akagi, Kohei Takeshita, Haruaki Hino, Rumi Hino, Ryosuke Kojima, Tasuku Ueno, Kenjiro Hanaoka, Seiichiro Abe, Yutaka Saito, Jun Nakajima, Yasuteru Urano

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Fluorescent probes that can selectively detect tumour lesions have great potential for fluorescence imaging-guided surgery. Here, we established a library-based approach for efficient screening of probes for tumour-selective imaging based on discovery of biomarker enzymes. We constructed a combinatorial fluorescent probe library for aminopeptidases and proteases, which is composed of 380 probes with various substrate moieties. Using this probe library, we performed lysate-based in vitro screening and/or direct imaging-based ex vivo screening of freshly resected clinical specimens from lung or gastric cancer patients, and found promising probes for tumour-selective visualization. Further, we identified two target enzymes as novel biomarker enzymes for discriminating between tumour and non-tumour tissues. This library-based approach is expected to be an efficient tool to develop tumour-imaging probes and to discover new biomarker enzyme activities for various tumours and other diseases.

Original language	English
Journal	Chemical Science
DOIs	https://doi.org/10.1039/d1sc06889j
Publication status	Accepted/In press - 2022
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/d1sc06889j

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Kuriki, Y., Yoshioka, T., Kamiya, M., Komatsu, T., Takamaru, H., Fujita, K., Iwaki, H., Nanjo, A., Akagi, Y., Takeshita, K., Hino, H., Hino, R., Kojima, R., Ueno, T., Hanaoka, K., Abe, S., Saito, Y., Nakajima, J., & Urano, Y. (Accepted/In press). Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities. Chemical Science. https://doi.org/10.1039/d1sc06889j

Kuriki, Y, Yoshioka, T, Kamiya, M, Komatsu, T, Takamaru, H, Fujita, K, Iwaki, H, Nanjo, A, Akagi, Y, Takeshita, K, Hino, H, Hino, R, Kojima, R, Ueno, T, Hanaoka, K, Abe, S, Saito, Y, Nakajima, J & Urano, Y 2022, 'Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities', Chemical Science. https://doi.org/10.1039/d1sc06889j

@article{61618e04e1c840f686c6da76157f18e9,

title = "Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities",

abstract = "Fluorescent probes that can selectively detect tumour lesions have great potential for fluorescence imaging-guided surgery. Here, we established a library-based approach for efficient screening of probes for tumour-selective imaging based on discovery of biomarker enzymes. We constructed a combinatorial fluorescent probe library for aminopeptidases and proteases, which is composed of 380 probes with various substrate moieties. Using this probe library, we performed lysate-based in vitro screening and/or direct imaging-based ex vivo screening of freshly resected clinical specimens from lung or gastric cancer patients, and found promising probes for tumour-selective visualization. Further, we identified two target enzymes as novel biomarker enzymes for discriminating between tumour and non-tumour tissues. This library-based approach is expected to be an efficient tool to develop tumour-imaging probes and to discover new biomarker enzyme activities for various tumours and other diseases.",

author = "Yugo Kuriki and Takafusa Yoshioka and Mako Kamiya and Toru Komatsu and Hiroyuki Takamaru and Kyohhei Fujita and Hirohisa Iwaki and Aika Nanjo and Yuki Akagi and Kohei Takeshita and Haruaki Hino and Rumi Hino and Ryosuke Kojima and Tasuku Ueno and Kenjiro Hanaoka and Seiichiro Abe and Yutaka Saito and Jun Nakajima and Yasuteru Urano",

note = "Funding Information: This research was supported in part by AMED under grant number JP19gm0710008 (to Y. U.), by JST grant Moonshot R&D MILLENNIA Program, JPMJMS2022-12 (to Y. U.), by JST/PRESTO grant JPMJPR14F8 (to M. K.) and 13414915 (to T. K.), by MEXT/JSPS KAKENHI grants JP19H05632 (to Y. U.) and JP19H02826 and JP20H05724 (to M. K.) and 19H02846 (to T. K.), by JSPS Core-to-Core Program (grant number JPJSCCA20170007, to Y. U.), A. Advanced Research Networks, by Japan Foundation for Applied Enzymology (to M. K.), and The Naito Foundation (to M. K.), as well as a JSPS stipend (to Y. K.). The authors thank Satoru Nonaka, Haruhisa Suzuki, Shigetaka Yoshinaga, Ichiro Oda for the collection of ESD specimens. Publisher Copyright: {\textcopyright} 2022 The Royal Society of Chemistry",

year = "2022",

doi = "10.1039/d1sc06889j",

language = "English",

journal = "Chemical Science",

issn = "2041-6520",

publisher = "Royal Society of Chemistry",

}

TY - JOUR

T1 - Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities

AU - Kuriki, Yugo

AU - Yoshioka, Takafusa

AU - Kamiya, Mako

AU - Komatsu, Toru

AU - Takamaru, Hiroyuki

AU - Fujita, Kyohhei

AU - Iwaki, Hirohisa

AU - Nanjo, Aika

AU - Akagi, Yuki

AU - Takeshita, Kohei

AU - Hino, Haruaki

AU - Hino, Rumi

AU - Kojima, Ryosuke

AU - Ueno, Tasuku

AU - Hanaoka, Kenjiro

AU - Abe, Seiichiro

AU - Saito, Yutaka

AU - Nakajima, Jun

AU - Urano, Yasuteru

N1 - Funding Information: This research was supported in part by AMED under grant number JP19gm0710008 (to Y. U.), by JST grant Moonshot R&D MILLENNIA Program, JPMJMS2022-12 (to Y. U.), by JST/PRESTO grant JPMJPR14F8 (to M. K.) and 13414915 (to T. K.), by MEXT/JSPS KAKENHI grants JP19H05632 (to Y. U.) and JP19H02826 and JP20H05724 (to M. K.) and 19H02846 (to T. K.), by JSPS Core-to-Core Program (grant number JPJSCCA20170007, to Y. U.), A. Advanced Research Networks, by Japan Foundation for Applied Enzymology (to M. K.), and The Naito Foundation (to M. K.), as well as a JSPS stipend (to Y. K.). The authors thank Satoru Nonaka, Haruhisa Suzuki, Shigetaka Yoshinaga, Ichiro Oda for the collection of ESD specimens. Publisher Copyright: © 2022 The Royal Society of Chemistry

PY - 2022

Y1 - 2022

N2 - Fluorescent probes that can selectively detect tumour lesions have great potential for fluorescence imaging-guided surgery. Here, we established a library-based approach for efficient screening of probes for tumour-selective imaging based on discovery of biomarker enzymes. We constructed a combinatorial fluorescent probe library for aminopeptidases and proteases, which is composed of 380 probes with various substrate moieties. Using this probe library, we performed lysate-based in vitro screening and/or direct imaging-based ex vivo screening of freshly resected clinical specimens from lung or gastric cancer patients, and found promising probes for tumour-selective visualization. Further, we identified two target enzymes as novel biomarker enzymes for discriminating between tumour and non-tumour tissues. This library-based approach is expected to be an efficient tool to develop tumour-imaging probes and to discover new biomarker enzyme activities for various tumours and other diseases.

AB - Fluorescent probes that can selectively detect tumour lesions have great potential for fluorescence imaging-guided surgery. Here, we established a library-based approach for efficient screening of probes for tumour-selective imaging based on discovery of biomarker enzymes. We constructed a combinatorial fluorescent probe library for aminopeptidases and proteases, which is composed of 380 probes with various substrate moieties. Using this probe library, we performed lysate-based in vitro screening and/or direct imaging-based ex vivo screening of freshly resected clinical specimens from lung or gastric cancer patients, and found promising probes for tumour-selective visualization. Further, we identified two target enzymes as novel biomarker enzymes for discriminating between tumour and non-tumour tissues. This library-based approach is expected to be an efficient tool to develop tumour-imaging probes and to discover new biomarker enzyme activities for various tumours and other diseases.

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U2 - 10.1039/d1sc06889j

DO - 10.1039/d1sc06889j

M3 - Article

AN - SCOPUS:85127683065

SN - 2041-6520

JO - Chemical Science

JF - Chemical Science

ER -

Development of a fluorescent probe library enabling efficient screening of tumour-imaging probes based on discovery of biomarker enzymatic activities

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