Development of a highly selective fluorescence probe for alkaline phosphatase

Mitsuyasu Kawaguchi, Kenjiro Hanaoka, Toru Komatsu, Takuya Terai, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We have developed the first highly selective fluorescence probe for alkaline phosphatase (ALP), TG-mPhos. This probe shows selectivity for ALP over protein tyrosine phosphatase and protein serine/threonine phosphatase. Our previously developed TG-Phos, which has a phenolic phosphate linkage in place of the alcoholic phosphate linkage of TG-mPhos, lacks this selectivity. TG-mPhos should enable precise fluorescence imaging of ALP activity in biological applications.

Original languageEnglish
Pages (from-to)5088-5091
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume21
Issue number17
DOIs
Publication statusPublished - 2011 Sep 1
Externally publishedYes

Keywords

  • Alkaline phosphatase
  • Fluorescence probe
  • Phosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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