TY - JOUR
T1 - Development of a highly selective fluorescence probe for alkaline phosphatase
AU - Kawaguchi, Mitsuyasu
AU - Hanaoka, Kenjiro
AU - Komatsu, Toru
AU - Terai, Takuya
AU - Nagano, Tetsuo
N1 - Funding Information:
This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant Nos. 22000006 to T.N., 20689001 and 21659024 to K.H., and 21750135 to T.T.), and by a grant from the Industrial Technology Development Organization (NEDO) of Japan (to T.T.). K.H. was also supported by Sankyo Foundation of Life Science and M.K. was supported by a Grant-in-Aid for JSPS Fellows. This work was supported in part by funds from the Target Protein Research Programs from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2011/9/1
Y1 - 2011/9/1
N2 - We have developed the first highly selective fluorescence probe for alkaline phosphatase (ALP), TG-mPhos. This probe shows selectivity for ALP over protein tyrosine phosphatase and protein serine/threonine phosphatase. Our previously developed TG-Phos, which has a phenolic phosphate linkage in place of the alcoholic phosphate linkage of TG-mPhos, lacks this selectivity. TG-mPhos should enable precise fluorescence imaging of ALP activity in biological applications.
AB - We have developed the first highly selective fluorescence probe for alkaline phosphatase (ALP), TG-mPhos. This probe shows selectivity for ALP over protein tyrosine phosphatase and protein serine/threonine phosphatase. Our previously developed TG-Phos, which has a phenolic phosphate linkage in place of the alcoholic phosphate linkage of TG-mPhos, lacks this selectivity. TG-mPhos should enable precise fluorescence imaging of ALP activity in biological applications.
KW - Alkaline phosphatase
KW - Fluorescence probe
KW - Phosphatase
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U2 - 10.1016/j.bmcl.2011.03.070
DO - 10.1016/j.bmcl.2011.03.070
M3 - Article
C2 - 21482108
AN - SCOPUS:80051801313
SN - 0960-894X
VL - 21
SP - 5088
EP - 5091
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 17
ER -