Development of a highly sensitive fluorescence probe for hydrogen peroxide

Masahiro Abo; Yasuteru Urano; Kenjiro Hanaoka; Takuya Terai; Toru Komatsu; Tetsuo Nagano

doi:10.1021/ja203521e

Development of a highly sensitive fluorescence probe for hydrogen peroxide

Masahiro Abo, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Toru Komatsu, Tetsuo Nagano

Research output: Contribution to journal › Article › peer-review

282 Citations (Scopus)

Abstract

Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.

Original language	English
Pages (from-to)	10629-10637
Number of pages	9
Journal	Journal of the American Chemical Society
Volume	133
Issue number	27
DOIs	https://doi.org/10.1021/ja203521e
Publication status	Published - 2011 Jul 13
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja203521e

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author = "Masahiro Abo and Yasuteru Urano and Kenjiro Hanaoka and Takuya Terai and Toru Komatsu and Tetsuo Nagano",

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AU - Abo, Masahiro

AU - Urano, Yasuteru

AU - Hanaoka, Kenjiro

AU - Terai, Takuya

AU - Komatsu, Toru

AU - Nagano, Tetsuo

PY - 2011/7/13

Y1 - 2011/7/13

N2 - Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.

AB - Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.

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Development of a highly sensitive fluorescence probe for hydrogen peroxide

Abstract

ASJC Scopus subject areas

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