Development of a highly sensitive fluorescence probe for hydrogen peroxide

Masahiro Abo, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Toru Komatsu, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

263 Citations (Scopus)

Abstract

Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.

Original languageEnglish
Pages (from-to)10629-10637
Number of pages9
JournalJournal of the American Chemical Society
Volume133
Issue number27
DOIs
Publication statusPublished - 2011 Jul 13
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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