Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

Yuji Kubo; Takashi Inaoka; Tsuyoshi Hachiya; Masayuki Miyake; Sumitaka Hase; Rikio Nakagawa; Hiromasa Hasegawa; Kazumi Funane; Yasubumi Sakakibara; Keitarou Kimura

doi:10.1016/j.jbiosc.2012.12.012

Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

Yuji Kubo, Takashi Inaoka, Tsuyoshi Hachiya, Masayuki Miyake, Sumitaka Hase, Rikio Nakagawa, Hiromasa Hasegawa, Kazumi Funane, Yasubumi Sakakibara, Keitarou Kimura

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.

Original language	English
Pages (from-to)	654-657
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	115
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2012.12.012
Publication status	Published - 2013 Jun

Keywords

Bacillus subtilis
Colored soybean
Exoenzyme production
Natto
Rifampicin resistance

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. / Kubo, Yuji; Inaoka, Takashi; Hachiya, Tsuyoshi; Miyake, Masayuki; Hase, Sumitaka; Nakagawa, Rikio; Hasegawa, Hiromasa; Funane, Kazumi; Sakakibara, Yasubumi; Kimura, Keitarou.

In: Journal of Bioscience and Bioengineering, Vol. 115, No. 6, 06.2013, p. 654-657.

Research output: Contribution to journal › Article › peer-review

Kubo, Yuji ; Inaoka, Takashi ; Hachiya, Tsuyoshi ; Miyake, Masayuki ; Hase, Sumitaka ; Nakagawa, Rikio ; Hasegawa, Hiromasa ; Funane, Kazumi ; Sakakibara, Yasubumi ; Kimura, Keitarou. / Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. In: Journal of Bioscience and Bioengineering. 2013 ; Vol. 115, No. 6. pp. 654-657.

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title = "Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production",

abstract = "The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.",

keywords = "Bacillus subtilis, Colored soybean, Exoenzyme production, Natto, Rifampicin resistance",

author = "Yuji Kubo and Takashi Inaoka and Tsuyoshi Hachiya and Masayuki Miyake and Sumitaka Hase and Rikio Nakagawa and Hiromasa Hasegawa and Kazumi Funane and Yasubumi Sakakibara and Keitarou Kimura",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for KAKENHI (Grant-in-Aid for Scientific Research) on Innovative Areas (no. 221S0002 ) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Daniel Hohlweck for critical reviewing of the manuscript. ",

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AU - Miyake, Masayuki

AU - Hase, Sumitaka

AU - Nakagawa, Rikio

AU - Hasegawa, Hiromasa

AU - Funane, Kazumi

AU - Sakakibara, Yasubumi

AU - Kimura, Keitarou

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KW - Bacillus subtilis

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