Abstract
The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.
| Original language | English |
|---|---|
| Pages (from-to) | 654-657 |
| Number of pages | 4 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 115 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2013 Jun |
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Keywords
- Bacillus subtilis
- Colored soybean
- Exoenzyme production
- Natto
- Rifampicin resistance
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. / Kubo, Yuji; Inaoka, Takashi; Hachiya, Tsuyoshi; Miyake, Masayuki; Hase, Sumitaka; Nakagawa, Rikio; Hasegawa, Hiromasa; Funane, Kazumi; Sakakibara, Yasubumi; Kimura, Keitarou.
In: Journal of Bioscience and Bioengineering, Vol. 115, No. 6, 06.2013, p. 654-657.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production
AU - Kubo, Yuji
AU - Inaoka, Takashi
AU - Hachiya, Tsuyoshi
AU - Miyake, Masayuki
AU - Hase, Sumitaka
AU - Nakagawa, Rikio
AU - Hasegawa, Hiromasa
AU - Funane, Kazumi
AU - Sakakibara, Yasubumi
AU - Kimura, Keitarou
PY - 2013/6
Y1 - 2013/6
N2 - The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.
AB - The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.
KW - Bacillus subtilis
KW - Colored soybean
KW - Exoenzyme production
KW - Natto
KW - Rifampicin resistance
UR - http://www.scopus.com/inward/record.url?scp=84876989970&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876989970&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2012.12.012
DO - 10.1016/j.jbiosc.2012.12.012
M3 - Article
VL - 115
SP - 654
EP - 657
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 6
ER -