Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

Yuji Kubo; Takashi Inaoka; Tsuyoshi Hachiya; Masayuki Miyake; Sumitaka Hase; Rikio Nakagawa; Hiromasa Hasegawa; Kazumi Funane; Yasubumi Sakakibara; Keitarou Kimura

doi:10.1016/j.jbiosc.2012.12.012

Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

Yuji Kubo, Takashi Inaoka, Tsuyoshi Hachiya, Masayuki Miyake, Sumitaka Hase, Rikio Nakagawa, Hiromasa Hasegawa, Kazumi Funane, Yasubumi Sakakibara, Keitarou Kimura

Department of Biosciences and Informatics

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.

Original language	English
Pages (from-to)	654-657
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	115
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2012.12.012
Publication status	Published - 2013 Jun

Keywords

Bacillus subtilis
Colored soybean
Exoenzyme production
Natto
Rifampicin resistance

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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Kubo, Y., Inaoka, T., Hachiya, T., Miyake, M., Hase, S., Nakagawa, R., Hasegawa, H., Funane, K., Sakakibara, Y., & Kimura, K. (2013). Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. Journal of Bioscience and Bioengineering, 115(6), 654-657. https://doi.org/10.1016/j.jbiosc.2012.12.012

Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. / Kubo, Yuji; Inaoka, Takashi; Hachiya, Tsuyoshi; Miyake, Masayuki; Hase, Sumitaka; Nakagawa, Rikio; Hasegawa, Hiromasa; Funane, Kazumi; Sakakibara, Yasubumi; Kimura, Keitarou.

In: Journal of Bioscience and Bioengineering, Vol. 115, No. 6, 06.2013, p. 654-657.

Research output: Contribution to journal › Article

Kubo, Yuji ; Inaoka, Takashi ; Hachiya, Tsuyoshi ; Miyake, Masayuki ; Hase, Sumitaka ; Nakagawa, Rikio ; Hasegawa, Hiromasa ; Funane, Kazumi ; Sakakibara, Yasubumi ; Kimura, Keitarou. / Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. In: Journal of Bioscience and Bioengineering. 2013 ; Vol. 115, No. 6. pp. 654-657.

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