TY - JOUR
T1 - Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production
AU - Kubo, Yuji
AU - Inaoka, Takashi
AU - Hachiya, Tsuyoshi
AU - Miyake, Masayuki
AU - Hase, Sumitaka
AU - Nakagawa, Rikio
AU - Hasegawa, Hiromasa
AU - Funane, Kazumi
AU - Sakakibara, Yasubumi
AU - Kimura, Keitarou
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for KAKENHI (Grant-in-Aid for Scientific Research) on Innovative Areas (no. 221S0002 ) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Daniel Hohlweck for critical reviewing of the manuscript.
PY - 2013/6
Y1 - 2013/6
N2 - The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.
AB - The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.
KW - Bacillus subtilis
KW - Colored soybean
KW - Exoenzyme production
KW - Natto
KW - Rifampicin resistance
UR - http://www.scopus.com/inward/record.url?scp=84876989970&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876989970&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2012.12.012
DO - 10.1016/j.jbiosc.2012.12.012
M3 - Article
C2 - 23318248
AN - SCOPUS:84876989970
VL - 115
SP - 654
EP - 657
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 6
ER -