Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

Yuji Kubo, Takashi Inaoka, Tsuyoshi Hachiya, Masayuki Miyake, Sumitaka Hase, Rikio Nakagawa, Hiromasa Hasegawa, Kazumi Funane, Yasubumi Sakakibara, Keitarou Kimura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.

Original languageEnglish
Pages (from-to)654-657
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume115
Issue number6
DOIs
Publication statusPublished - 2013 Jun

Fingerprint

levansucrase
Bacilli
DNA-Directed RNA Polymerases
Rifampin
Bacillus subtilis
Proteomics
Fermentation
Peptide Hydrolases
Phenotype
Mutation
Starters
RNA

Keywords

  • Bacillus subtilis
  • Colored soybean
  • Exoenzyme production
  • Natto
  • Rifampicin resistance

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. / Kubo, Yuji; Inaoka, Takashi; Hachiya, Tsuyoshi; Miyake, Masayuki; Hase, Sumitaka; Nakagawa, Rikio; Hasegawa, Hiromasa; Funane, Kazumi; Sakakibara, Yasubumi; Kimura, Keitarou.

In: Journal of Bioscience and Bioengineering, Vol. 115, No. 6, 06.2013, p. 654-657.

Research output: Contribution to journalArticle

Kubo, Yuji ; Inaoka, Takashi ; Hachiya, Tsuyoshi ; Miyake, Masayuki ; Hase, Sumitaka ; Nakagawa, Rikio ; Hasegawa, Hiromasa ; Funane, Kazumi ; Sakakibara, Yasubumi ; Kimura, Keitarou. / Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. In: Journal of Bioscience and Bioengineering. 2013 ; Vol. 115, No. 6. pp. 654-657.
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AU - Hase, Sumitaka

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