Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

Yuji Kubo, Takashi Inaoka, Tsuyoshi Hachiya, Masayuki Miyake, Sumitaka Hase, Rikio Nakagawa, Hiromasa Hasegawa, Kazumi Funane, Yasubumi Sakakibara, Keitarou Kimura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.

Original languageEnglish
Pages (from-to)654-657
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume115
Issue number6
DOIs
Publication statusPublished - 2013 Jun

Keywords

  • Bacillus subtilis
  • Colored soybean
  • Exoenzyme production
  • Natto
  • Rifampicin resistance

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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