Development of a sensitive bioluminogenic probe for imaging highly reactive oxygen species in living rats

Ryosuke Kojima, Hideo Takakura, Mako Kamiya, Eiji Kobayashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

A sensitive bioluminogenic probe for highly reactive oxygen species (hROS), SO3H-APL, was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme-induced electron transfer (BioLeT) and modulation of cell-membrane permeability. This probe enables non-invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats for the first time. It is expected to serve as a practical analytical tool for investigating a wide range of biological functions of hROS in vivo. The design concept should be applicable to other in vivo bioluminogenic probes. You light up my life: A sensitive bioluminogenic probe for highly reactive oxygen species (hROS) was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme-induced electron transfer (BioLeT) and modulation of cell-membrane permeability. This probe enables non-invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats.

Original languageEnglish
Pages (from-to)14768-14771
Number of pages4
JournalAngewandte Chemie - International Edition
Volume54
Issue number49
DOIs
Publication statusPublished - 2015 Dec 1
Externally publishedYes

Keywords

  • electron transfer
  • imaging agents
  • luminescence
  • membrane permeability
  • reactive oxygen species

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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