Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus

Yasuko Tsunetsugu-Yokota, Kengo Nishimura, Shuhei Misawa, Mie Kobayashi-Ishihara, Hitoshi Takahashi, Ikuyo Takayama, Kazuo Ohnishi, Shigeyuki Itamura, Hang L.K. Nguyen, Mai T.Q. Le, Giang T. Dang, Long T. Nguyen, Masato Tashiro, Tsutomu Kageyama

Research output: Contribution to journalArticle

6 Citations (Scopus)


Background: Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed.Methods: By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube.Resutls: We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit.Conclusions: This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.

Original languageEnglish
Article number362
JournalBMC infectious diseases
Issue number1
Publication statusPublished - 2014 Jul 3
Externally publishedYes



  • Clinical specimens
  • H5 hemagglutinin
  • Highly pathogenic avian influenza virus
  • Monoclonal antibody
  • Rapid influenza diagnosis

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Tsunetsugu-Yokota, Y., Nishimura, K., Misawa, S., Kobayashi-Ishihara, M., Takahashi, H., Takayama, I., Ohnishi, K., Itamura, S., Nguyen, H. L. K., Le, M. T. Q., Dang, G. T., Nguyen, L. T., Tashiro, M., & Kageyama, T. (2014). Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus. BMC infectious diseases, 14(1), [362].