Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus

Yasuko Tsunetsugu-Yokota, Kengo Nishimura, Shuhei Misawa, Mie Kobayashi-Ishihara, Hitoshi Takahashi, Ikuyo Takayama, Kazuo Ohnishi, Shigeyuki Itamura, Hang L.K. Nguyen, Mai T.Q. Le, Giang T. Dang, Long T. Nguyen, Masato Tashiro, Tsutomu Kageyama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed.Methods: By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube.Resutls: We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit.Conclusions: This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.

Original languageEnglish
Article number362
JournalBMC infectious diseases
Volume14
Issue number1
DOIs
Publication statusPublished - 2014 Jul 3
Externally publishedYes

Fingerprint

H5N1 Subtype Influenza A Virus
Influenza in Birds
Influenza A virus
Orthomyxoviridae
Virus Diseases
Luminescence
Human Influenza
Immunochromatography
Vietnam
Pandemics
Immunoenzyme Techniques
Monoclonal Antibodies
Technology
Antigens
Polymerase Chain Reaction

Keywords

  • Clinical specimens
  • H5 hemagglutinin
  • Highly pathogenic avian influenza virus
  • Monoclonal antibody
  • Rapid influenza diagnosis

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus. / Tsunetsugu-Yokota, Yasuko; Nishimura, Kengo; Misawa, Shuhei; Kobayashi-Ishihara, Mie; Takahashi, Hitoshi; Takayama, Ikuyo; Ohnishi, Kazuo; Itamura, Shigeyuki; Nguyen, Hang L.K.; Le, Mai T.Q.; Dang, Giang T.; Nguyen, Long T.; Tashiro, Masato; Kageyama, Tsutomu.

In: BMC infectious diseases, Vol. 14, No. 1, 362, 03.07.2014.

Research output: Contribution to journalArticle

Tsunetsugu-Yokota, Y, Nishimura, K, Misawa, S, Kobayashi-Ishihara, M, Takahashi, H, Takayama, I, Ohnishi, K, Itamura, S, Nguyen, HLK, Le, MTQ, Dang, GT, Nguyen, LT, Tashiro, M & Kageyama, T 2014, 'Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus', BMC infectious diseases, vol. 14, no. 1, 362. https://doi.org/10.1186/1471-2334-14-362
Tsunetsugu-Yokota, Yasuko ; Nishimura, Kengo ; Misawa, Shuhei ; Kobayashi-Ishihara, Mie ; Takahashi, Hitoshi ; Takayama, Ikuyo ; Ohnishi, Kazuo ; Itamura, Shigeyuki ; Nguyen, Hang L.K. ; Le, Mai T.Q. ; Dang, Giang T. ; Nguyen, Long T. ; Tashiro, Masato ; Kageyama, Tsutomu. / Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus. In: BMC infectious diseases. 2014 ; Vol. 14, No. 1.
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AU - Kobayashi-Ishihara, Mie

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AU - Ohnishi, Kazuo

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