Abstract
Ion behavior confined in extended nanospace (101-103 nm) is important to develop novel miniaturized systems for biochemical analysis and further applications. The present study developed a measurement technique of ion distribution in nanochannel using stimulated emission depletion microscopy to achieve a spatial resolution of 87 nm. Fluorescein was used for ratiometric measurement of pH by two-excitation wavelengths. The proton distribution in a 2D nanochannel of 410 nm width and 405 nm depth was firstly measured at an uncertainty of pH 0.05, which was strongly related to the electric double layer. This technique will greatly contribute to establish nanofluidics and nanochemistry.
| Original language | English |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 1251-1253 |
| Number of pages | 3 |
| Publication status | Published - 2011 Dec 1 |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 2011 Oct 2 → 2011 Oct 6 |
Publication series
| Name | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Volume | 2 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Country | United States |
| City | Seattle, WA |
| Period | 11/10/2 → 11/10/6 |
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Keywords
- Extendednanochannel
- Fluorescence
- Proton
- Stimulated emission depletion
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Development of a visualization technique of proton concentration in extended nanospace channel using stimulated emission depletion microscopy. / Kazoe, Yutaka; Mawatari, K.; Sugii, Y.; Kitamori, T.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1251-1253 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Development of a visualization technique of proton concentration in extended nanospace channel using stimulated emission depletion microscopy
AU - Kazoe, Yutaka
AU - Mawatari, K.
AU - Sugii, Y.
AU - Kitamori, T.
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Ion behavior confined in extended nanospace (101-103 nm) is important to develop novel miniaturized systems for biochemical analysis and further applications. The present study developed a measurement technique of ion distribution in nanochannel using stimulated emission depletion microscopy to achieve a spatial resolution of 87 nm. Fluorescein was used for ratiometric measurement of pH by two-excitation wavelengths. The proton distribution in a 2D nanochannel of 410 nm width and 405 nm depth was firstly measured at an uncertainty of pH 0.05, which was strongly related to the electric double layer. This technique will greatly contribute to establish nanofluidics and nanochemistry.
AB - Ion behavior confined in extended nanospace (101-103 nm) is important to develop novel miniaturized systems for biochemical analysis and further applications. The present study developed a measurement technique of ion distribution in nanochannel using stimulated emission depletion microscopy to achieve a spatial resolution of 87 nm. Fluorescein was used for ratiometric measurement of pH by two-excitation wavelengths. The proton distribution in a 2D nanochannel of 410 nm width and 405 nm depth was firstly measured at an uncertainty of pH 0.05, which was strongly related to the electric double layer. This technique will greatly contribute to establish nanofluidics and nanochemistry.
KW - Extendednanochannel
KW - Fluorescence
KW - Proton
KW - Stimulated emission depletion
UR - http://www.scopus.com/inward/record.url?scp=84883796160&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883796160&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883796160
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1251
EP - 1253
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ER -