Development of a visualization technique of proton concentration in extended nanospace channel using stimulated emission depletion microscopy

Y. Kazoe, K. Mawatari, Y. Sugii, T. Kitamori

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Ion behavior confined in extended nanospace (101-103 nm) is important to develop novel miniaturized systems for biochemical analysis and further applications. The present study developed a measurement technique of ion distribution in nanochannel using stimulated emission depletion microscopy to achieve a spatial resolution of 87 nm. Fluorescein was used for ratiometric measurement of pH by two-excitation wavelengths. The proton distribution in a 2D nanochannel of 410 nm width and 405 nm depth was firstly measured at an uncertainty of pH 0.05, which was strongly related to the electric double layer. This technique will greatly contribute to establish nanofluidics and nanochemistry.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1251-1253
Number of pages3
Publication statusPublished - 2011 Dec 1
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • Extendednanochannel
  • Fluorescence
  • Proton
  • Stimulated emission depletion

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Kazoe, Y., Mawatari, K., Sugii, Y., & Kitamori, T. (2011). Development of a visualization technique of proton concentration in extended nanospace channel using stimulated emission depletion microscopy. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1251-1253). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).