Development of acquired immunity following repeated respiratory syncytial virus infections in cotton rats

Yoshiaki Yamaji, Yosuke Yasui, Tetsuo Nakayama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Respiratory syncytial virus (RSV) infections occur every year worldwide. Most infants are infected with RSV by one year of age and are reinfected because immune responses after the first infection are too weak to protect against subsequent infections. In the present study, immune responses against RSV were investigated in order to obtain a better understanding of repetitive RSV infections in cotton rats. No detectable neutralizing antibody (NT) was developed after the first infection, and the second infection was not prevented. The results of histological examinations revealed severe inflammation, viral antigens were detected around bronchial epithelial cells, and infectious viruses were recovered from lung homogenates. Following the second infection neutralizing antibodies were significantly elevated, and CD8+ cells were activated in response to RSV-F253-265. No viral antigens was detected thereafter in lung tissues and infectious viruses were not recovered. Similar results were obtained in the present study using the subgroups A and B. These results support the induction of humoral and cellular immune responses following repetitive infections with RSV; however, these responses were insufficient to eliminate viruses in the first and second infections.

Original languageEnglish
Article numbere0155777
JournalPLoS One
Volume11
Issue number5
DOIs
Publication statusPublished - 2016 May 1

Fingerprint

Sigmodon
Sigmodontinae
Respiratory Syncytial Virus Infections
Adaptive Immunity
Viruses
Cotton
Rats
viruses
Respiratory Syncytial Viruses
Infection
infection
Viral Antigens
Neutralizing Antibodies
viral antigens
neutralizing antibodies
Lung
Humoral Immunity
lungs
immune response
Cellular Immunity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Development of acquired immunity following repeated respiratory syncytial virus infections in cotton rats. / Yamaji, Yoshiaki; Yasui, Yosuke; Nakayama, Tetsuo.

In: PLoS One, Vol. 11, No. 5, e0155777, 01.05.2016.

Research output: Contribution to journalArticle

@article{13ece465af2541118b922cc1e355ed50,
title = "Development of acquired immunity following repeated respiratory syncytial virus infections in cotton rats",
abstract = "Respiratory syncytial virus (RSV) infections occur every year worldwide. Most infants are infected with RSV by one year of age and are reinfected because immune responses after the first infection are too weak to protect against subsequent infections. In the present study, immune responses against RSV were investigated in order to obtain a better understanding of repetitive RSV infections in cotton rats. No detectable neutralizing antibody (NT) was developed after the first infection, and the second infection was not prevented. The results of histological examinations revealed severe inflammation, viral antigens were detected around bronchial epithelial cells, and infectious viruses were recovered from lung homogenates. Following the second infection neutralizing antibodies were significantly elevated, and CD8+ cells were activated in response to RSV-F253-265. No viral antigens was detected thereafter in lung tissues and infectious viruses were not recovered. Similar results were obtained in the present study using the subgroups A and B. These results support the induction of humoral and cellular immune responses following repetitive infections with RSV; however, these responses were insufficient to eliminate viruses in the first and second infections.",
author = "Yoshiaki Yamaji and Yosuke Yasui and Tetsuo Nakayama",
year = "2016",
month = "5",
day = "1",
doi = "10.1371/journal.pone.0155777",
language = "English",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

TY - JOUR

T1 - Development of acquired immunity following repeated respiratory syncytial virus infections in cotton rats

AU - Yamaji, Yoshiaki

AU - Yasui, Yosuke

AU - Nakayama, Tetsuo

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Respiratory syncytial virus (RSV) infections occur every year worldwide. Most infants are infected with RSV by one year of age and are reinfected because immune responses after the first infection are too weak to protect against subsequent infections. In the present study, immune responses against RSV were investigated in order to obtain a better understanding of repetitive RSV infections in cotton rats. No detectable neutralizing antibody (NT) was developed after the first infection, and the second infection was not prevented. The results of histological examinations revealed severe inflammation, viral antigens were detected around bronchial epithelial cells, and infectious viruses were recovered from lung homogenates. Following the second infection neutralizing antibodies were significantly elevated, and CD8+ cells were activated in response to RSV-F253-265. No viral antigens was detected thereafter in lung tissues and infectious viruses were not recovered. Similar results were obtained in the present study using the subgroups A and B. These results support the induction of humoral and cellular immune responses following repetitive infections with RSV; however, these responses were insufficient to eliminate viruses in the first and second infections.

AB - Respiratory syncytial virus (RSV) infections occur every year worldwide. Most infants are infected with RSV by one year of age and are reinfected because immune responses after the first infection are too weak to protect against subsequent infections. In the present study, immune responses against RSV were investigated in order to obtain a better understanding of repetitive RSV infections in cotton rats. No detectable neutralizing antibody (NT) was developed after the first infection, and the second infection was not prevented. The results of histological examinations revealed severe inflammation, viral antigens were detected around bronchial epithelial cells, and infectious viruses were recovered from lung homogenates. Following the second infection neutralizing antibodies were significantly elevated, and CD8+ cells were activated in response to RSV-F253-265. No viral antigens was detected thereafter in lung tissues and infectious viruses were not recovered. Similar results were obtained in the present study using the subgroups A and B. These results support the induction of humoral and cellular immune responses following repetitive infections with RSV; however, these responses were insufficient to eliminate viruses in the first and second infections.

UR - http://www.scopus.com/inward/record.url?scp=84971277835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84971277835&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0155777

DO - 10.1371/journal.pone.0155777

M3 - Article

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 5

M1 - e0155777

ER -