Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections

Atsushi Matsuda, Atsushi Kuno, Hiroyasu Ishida, Toru Kawamoto, Jun ichi Shoda, Jun Hirabayashi

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

An ultra-sensitive method for glycan analysis targeting small tissue sections (1.5 mm in diameter) is described as an application of a recently-established lectin microarray technology. The developed system achieved a high level of detection of a tissue section consisting of approximately 500 cells for differential profiling, where both N- and O-glycans attached to a pool of glycoproteins are subjected to multiplex analysis with 43 lectins. By using an optimized protocol for differential glycan analysis, sections of adenocarcinoma (n = 28) and normal epithelia (n = 12) of the colon were analyzed in an all-in-one manner. As a result, Wisteria floribunda agglutinin (WFA) was found to clearly differentiate cancerous from normal epithelia with P < 0.0001. The obtained results correlated well with the subsequent histochemical study using biotinylated WFA. Thus, the developed technology proved to be valid for expanding the lectin microarray applications to tissue-based glycomics, and hence, should accelerate a discovery phase of glycan-related biomarkers.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume370
Issue number2
DOIs
Publication statusPublished - 2008 May 30
Externally publishedYes

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Keywords

  • Biomarker
  • Colon cancer
  • Formalin-embedded
  • Glycan profiling
  • Lectin microarray
  • Tissue array

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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