Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections

Atsushi Matsuda, Atsushi Kuno, Hiroyasu Ishida, Toru Kawamoto, Jun ichi Shoda, Jun Hirabayashi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

An ultra-sensitive method for glycan analysis targeting small tissue sections (1.5 mm in diameter) is described as an application of a recently-established lectin microarray technology. The developed system achieved a high level of detection of a tissue section consisting of approximately 500 cells for differential profiling, where both N- and O-glycans attached to a pool of glycoproteins are subjected to multiplex analysis with 43 lectins. By using an optimized protocol for differential glycan analysis, sections of adenocarcinoma (n = 28) and normal epithelia (n = 12) of the colon were analyzed in an all-in-one manner. As a result, Wisteria floribunda agglutinin (WFA) was found to clearly differentiate cancerous from normal epithelia with P < 0.0001. The obtained results correlated well with the subsequent histochemical study using biotinylated WFA. Thus, the developed technology proved to be valid for expanding the lectin microarray applications to tissue-based glycomics, and hence, should accelerate a discovery phase of glycan-related biomarkers.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume370
Issue number2
DOIs
Publication statusPublished - 2008 May 30
Externally publishedYes

Fingerprint

Formaldehyde
Polysaccharides
Lectins
Tissue
Technology
Microarrays
Epithelium
Glycomics
Biomarkers
Glycoproteins
Colon
Adenocarcinoma
wisteria lectin

Keywords

  • Biomarker
  • Colon cancer
  • Formalin-embedded
  • Glycan profiling
  • Lectin microarray
  • Tissue array

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections. / Matsuda, Atsushi; Kuno, Atsushi; Ishida, Hiroyasu; Kawamoto, Toru; Shoda, Jun ichi; Hirabayashi, Jun.

In: Biochemical and Biophysical Research Communications, Vol. 370, No. 2, 30.05.2008, p. 259-263.

Research output: Contribution to journalArticle

Matsuda, Atsushi ; Kuno, Atsushi ; Ishida, Hiroyasu ; Kawamoto, Toru ; Shoda, Jun ichi ; Hirabayashi, Jun. / Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 370, No. 2. pp. 259-263.
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