Development of an antigen-specific CD8 suppressor effector clone in man

Tsutomu Takeuchi, S. F. Schlossman, C. Morimoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A long-term cultured IL-2-dependent keyhole limpet hemocyanin (KLH)-specific CD8 (T8) suppressor clone (5B9) was generated from a healthy donor hyperimmunized with KLH. The 5B9 clonal population suppressed in vitro anti-KLH antibody response but did not suppress anti-TT antibody response or PWM-driven IgG synthesis. Moreover, 5B9 cells could not suppress anti-TT antibody response even in the presence of free KLH. 5B9 cloned cells suppressed the anti-KLH antibody response of B cells cultured with CD4+4B4+ cells without requiring the presence of CD8+ cells. This KLH-specific CD8 suppressor clone is an effector type rather than an inducer type of suppressor T cell. The cloned cells expressed α- and β-TCR proteins (defined by WT-31 antibody) on their cell surface. More importantly, the CD3:TCR complex was functionally important in the suppression induced by this clone, because after CD3 antigen modulation from its cell surface, the suppressor effector function was abolished.

Original languageEnglish
Pages (from-to)3010-3015
Number of pages6
JournalJournal of Immunology
Volume141
Issue number9
Publication statusPublished - 1988
Externally publishedYes

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CD8 Antigens
Clone Cells
Antibody Formation
Anti-Idiotypic Antibodies
T-Cell Antigen Receptor-CD3 Complex
CD3 Antigens
Interleukin-2
keyhole-limpet hemocyanin
B-Lymphocytes
Immunoglobulin G
Tissue Donors
T-Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Development of an antigen-specific CD8 suppressor effector clone in man. / Takeuchi, Tsutomu; Schlossman, S. F.; Morimoto, C.

In: Journal of Immunology, Vol. 141, No. 9, 1988, p. 3010-3015.

Research output: Contribution to journalArticle

Takeuchi, T, Schlossman, SF & Morimoto, C 1988, 'Development of an antigen-specific CD8 suppressor effector clone in man', Journal of Immunology, vol. 141, no. 9, pp. 3010-3015.
Takeuchi, Tsutomu ; Schlossman, S. F. ; Morimoto, C. / Development of an antigen-specific CD8 suppressor effector clone in man. In: Journal of Immunology. 1988 ; Vol. 141, No. 9. pp. 3010-3015.
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