TY - JOUR
T1 - Development of an automated large-scale protein-crystallization and monitoring system for high-throughput protein-structure analyses
AU - Hiraki, Masahiko
AU - Kato, Ryuichi
AU - Nagai, Minoru
AU - Satoh, Tadashi
AU - Hirano, Satoshi
AU - Ihara, Kentaro
AU - Kudo, Norio
AU - Nagae, Masamichi
AU - Kobayashi, Masanori
AU - Inoue, Michio
AU - Uejima, Tamami
AU - Oda, Shunichiro
AU - Chavas, Leonard M.G.
AU - Akutsu, Masato
AU - Yamada, Yusuke
AU - Kawasaki, Masato
AU - Matsugaki, Naohiro
AU - Igarashi, Noriyuki
AU - Suzuki, Mamoru
AU - Wakatsuki, Soichi
PY - 2006/9
Y1 - 2006/9
N2 - Protein crystallization remains one of the bottlenecks in crystallographic analysis of macromolecules. An automated large-scale protein-crystallization system named PXS has been developed consisting of the following subsystems, which proceed in parallel under unified control software: dispensing precipitants and protein solutions, sealing crystallization plates, carrying robot, incubators, observation system and image-storage server. A sitting-drop crystallization plate specialized for PXS has also been designed and developed. PXS can set up 7680 drops for vapour diffusion per hour, which includes time for replenishing supplies such as disposable tips and crystallization plates. Images of the crystallization drops are automatically recorded according to a preprogrammed schedule and can be viewed by users remotely using web-based browser software. A number of protein crystals were successfully produced and several protein structures could be determined directly from crystals grown by PXS. In other cases, X-ray quality crystals were obtained by further optimization by manual screening based on the conditions found by PXS.
AB - Protein crystallization remains one of the bottlenecks in crystallographic analysis of macromolecules. An automated large-scale protein-crystallization system named PXS has been developed consisting of the following subsystems, which proceed in parallel under unified control software: dispensing precipitants and protein solutions, sealing crystallization plates, carrying robot, incubators, observation system and image-storage server. A sitting-drop crystallization plate specialized for PXS has also been designed and developed. PXS can set up 7680 drops for vapour diffusion per hour, which includes time for replenishing supplies such as disposable tips and crystallization plates. Images of the crystallization drops are automatically recorded according to a preprogrammed schedule and can be viewed by users remotely using web-based browser software. A number of protein crystals were successfully produced and several protein structures could be determined directly from crystals grown by PXS. In other cases, X-ray quality crystals were obtained by further optimization by manual screening based on the conditions found by PXS.
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U2 - 10.1107/S0907444906023821
DO - 10.1107/S0907444906023821
M3 - Article
C2 - 16929107
AN - SCOPUS:33748370297
SN - 0907-4449
VL - 62
SP - 1058
EP - 1065
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
IS - 9
ER -