Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging

Yuichiro Koide, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

436 Citations (Scopus)

Abstract

A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl.

Original languageEnglish
Pages (from-to)5680-5682
Number of pages3
JournalJournal of the American Chemical Society
Volume133
Issue number15
DOIs
Publication statusPublished - 2011 Apr 20
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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