Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging

Yuichiro Koide; Yasuteru Urano; Kenjiro Hanaoka; Takuya Terai; Tetsuo Nagano

doi:10.1021/ja111470n

Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging

Yuichiro Koide, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano

Research output: Contribution to journal › Article › peer-review

436 Citations (Scopus)

Abstract

A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl.

Original language	English
Pages (from-to)	5680-5682
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	133
Issue number	15
DOIs	https://doi.org/10.1021/ja111470n
Publication status	Published - 2011 Apr 20
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja111470n

Cite this

Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging. / Koide, Yuichiro; Urano, Yasuteru; Hanaoka, Kenjiro; Terai, Takuya; Nagano, Tetsuo.

In: Journal of the American Chemical Society, Vol. 133, No. 15, 20.04.2011, p. 5680-5682.

Research output: Contribution to journal › Article › peer-review

@article{7ec4aaa0b09443c89ae5fea551c2d995,

title = "Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging",

abstract = "A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl.",

author = "Yuichiro Koide and Yasuteru Urano and Kenjiro Hanaoka and Takuya Terai and Tetsuo Nagano",

year = "2011",

month = apr,

day = "20",

doi = "10.1021/ja111470n",

language = "English",

volume = "133",

pages = "5680--5682",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "15",

}

TY - JOUR

T1 - Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging

AU - Koide, Yuichiro

AU - Urano, Yasuteru

AU - Hanaoka, Kenjiro

AU - Terai, Takuya

AU - Nagano, Tetsuo

PY - 2011/4/20

Y1 - 2011/4/20

N2 - A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl.

AB - A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl.

UR - http://www.scopus.com/inward/record.url?scp=79954486027&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954486027&partnerID=8YFLogxK

U2 - 10.1021/ja111470n

DO - 10.1021/ja111470n

M3 - Article

C2 - 21443186

AN - SCOPUS:79954486027

VL - 133

SP - 5680

EP - 5682

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 15

ER -

Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this