Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging

Yuichiro Koide; Yasuteru Urano; Kenjiro Hanaoka; Takuya Terai; Tetsuo Nagano

doi:10.1021/ja111470n

Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging

Yuichiro Koide, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano

Research output: Contribution to journal › Article › peer-review

485 Citations (Scopus)

Abstract

A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl.

Original language	English
Pages (from-to)	5680-5682
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	133
Issue number	15
DOIs	https://doi.org/10.1021/ja111470n
Publication status	Published - 2011 Apr 20
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja111470n

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Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging. / Koide, Yuichiro; Urano, Yasuteru; Hanaoka, Kenjiro et al.

In: Journal of the American Chemical Society, Vol. 133, No. 15, 20.04.2011, p. 5680-5682.

Research output: Contribution to journal › Article › peer-review

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abstract = "A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl.",

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AU - Terai, Takuya

AU - Nagano, Tetsuo

PY - 2011/4/20

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Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging

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