Development of bioreactor for regenerative medicine and effect of mechanical stimuli on mesenchymal stem cells in polyurethane scaffolds

Min Jin Joo, Heoung Jae Chun, Hyung Jin Jung, Chang Gun Lee, Dong Nyoung Heo, Il Keun Kwon, Seong Hwan Moon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It is well known that mesenchymal stem cell (MSCs) can be differentiated into fibroblasts, chondrocytes, and osteoblasts and that they develop into fibrous tissue, cartilage, or bone, as a result of mechanical stimulation. In this study, we developed a bioreactor system, which is composed of a reactor vessel that provides the required cell culture environment, an environment controlling chamber to control the media, a gas mixer, and a reactor motion control subsystem to apply mechanical stimuli to the cells. For the MSC culture, We used a poly-urethane (PU) scaffold, with a collagen coating to ensure improved cohesion ratio. Then, we transferred the cultivated MSCs in the PU scaffold, cultured the cells in the bioreactor system, and confirmed the proliferation, differentiation, and ossification processes, resulting from mechanical stimuli.

Original languageEnglish
Pages (from-to)675-681
Number of pages7
JournalTransactions of the Korean Society of Mechanical Engineers, A
Volume34
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1

Keywords

  • Bioreactor
  • Mechanical stimulation
  • Mesenchymal stem cell
  • Polyurethane scaffold

ASJC Scopus subject areas

  • Mechanical Engineering

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