Development of bioreactor for regenerative medicine and effect of mechanical stimuli on mesenchymal stem cells in polyurethane scaffolds

Min Jin Joo, Heoung Jae Chun, Hyung Jin Jung, Chang Gun Lee, Dong Nyoung Heo, Il Keun Kwon, Seong Hwan Moon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It is well known that mesenchymal stem cell (MSCs) can be differentiated into fibroblasts, chondrocytes, and osteoblasts and that they develop into fibrous tissue, cartilage, or bone, as a result of mechanical stimulation. In this study, we developed a bioreactor system, which is composed of a reactor vessel that provides the required cell culture environment, an environment controlling chamber to control the media, a gas mixer, and a reactor motion control subsystem to apply mechanical stimuli to the cells. For the MSC culture, We used a poly-urethane (PU) scaffold, with a collagen coating to ensure improved cohesion ratio. Then, we transferred the cultivated MSCs in the PU scaffold, cultured the cells in the bioreactor system, and confirmed the proliferation, differentiation, and ossification processes, resulting from mechanical stimuli.

Original languageEnglish
Pages (from-to)675-681
Number of pages7
JournalTransactions of the Korean Society of Mechanical Engineers, A
Volume34
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1
Externally publishedYes

Fingerprint

Bioreactors
Stem cells
Scaffolds
Polyurethanes
Cell culture
Osteoblasts
Cartilage
Motion control
Fibroblasts
Scaffolds (biology)
Collagen
Bone
Tissue
Coatings
Gases
Regenerative Medicine

Keywords

  • Bioreactor
  • Mechanical stimulation
  • Mesenchymal stem cell
  • Polyurethane scaffold

ASJC Scopus subject areas

  • Mechanical Engineering

Cite this

Development of bioreactor for regenerative medicine and effect of mechanical stimuli on mesenchymal stem cells in polyurethane scaffolds. / Joo, Min Jin; Chun, Heoung Jae; Jung, Hyung Jin; Lee, Chang Gun; Heo, Dong Nyoung; Kwon, Il Keun; Moon, Seong Hwan.

In: Transactions of the Korean Society of Mechanical Engineers, A, Vol. 34, No. 6, 01.06.2010, p. 675-681.

Research output: Contribution to journalArticle

Joo, Min Jin ; Chun, Heoung Jae ; Jung, Hyung Jin ; Lee, Chang Gun ; Heo, Dong Nyoung ; Kwon, Il Keun ; Moon, Seong Hwan. / Development of bioreactor for regenerative medicine and effect of mechanical stimuli on mesenchymal stem cells in polyurethane scaffolds. In: Transactions of the Korean Society of Mechanical Engineers, A. 2010 ; Vol. 34, No. 6. pp. 675-681.
@article{5875a7cf2d564d50a6e1b08931903bb4,
title = "Development of bioreactor for regenerative medicine and effect of mechanical stimuli on mesenchymal stem cells in polyurethane scaffolds",
abstract = "It is well known that mesenchymal stem cell (MSCs) can be differentiated into fibroblasts, chondrocytes, and osteoblasts and that they develop into fibrous tissue, cartilage, or bone, as a result of mechanical stimulation. In this study, we developed a bioreactor system, which is composed of a reactor vessel that provides the required cell culture environment, an environment controlling chamber to control the media, a gas mixer, and a reactor motion control subsystem to apply mechanical stimuli to the cells. For the MSC culture, We used a poly-urethane (PU) scaffold, with a collagen coating to ensure improved cohesion ratio. Then, we transferred the cultivated MSCs in the PU scaffold, cultured the cells in the bioreactor system, and confirmed the proliferation, differentiation, and ossification processes, resulting from mechanical stimuli.",
keywords = "Bioreactor, Mechanical stimulation, Mesenchymal stem cell, Polyurethane scaffold",
author = "Joo, {Min Jin} and Chun, {Heoung Jae} and Jung, {Hyung Jin} and Lee, {Chang Gun} and Heo, {Dong Nyoung} and Kwon, {Il Keun} and Moon, {Seong Hwan}",
year = "2010",
month = "6",
day = "1",
doi = "10.3795/KSME-A.2010.34.6.675",
language = "English",
volume = "34",
pages = "675--681",
journal = "Transactions of the Korean Society of Mechanical Engineers, A",
issn = "1226-4873",
publisher = "Korean Society of Mechanical Engineers",
number = "6",

}

TY - JOUR

T1 - Development of bioreactor for regenerative medicine and effect of mechanical stimuli on mesenchymal stem cells in polyurethane scaffolds

AU - Joo, Min Jin

AU - Chun, Heoung Jae

AU - Jung, Hyung Jin

AU - Lee, Chang Gun

AU - Heo, Dong Nyoung

AU - Kwon, Il Keun

AU - Moon, Seong Hwan

PY - 2010/6/1

Y1 - 2010/6/1

N2 - It is well known that mesenchymal stem cell (MSCs) can be differentiated into fibroblasts, chondrocytes, and osteoblasts and that they develop into fibrous tissue, cartilage, or bone, as a result of mechanical stimulation. In this study, we developed a bioreactor system, which is composed of a reactor vessel that provides the required cell culture environment, an environment controlling chamber to control the media, a gas mixer, and a reactor motion control subsystem to apply mechanical stimuli to the cells. For the MSC culture, We used a poly-urethane (PU) scaffold, with a collagen coating to ensure improved cohesion ratio. Then, we transferred the cultivated MSCs in the PU scaffold, cultured the cells in the bioreactor system, and confirmed the proliferation, differentiation, and ossification processes, resulting from mechanical stimuli.

AB - It is well known that mesenchymal stem cell (MSCs) can be differentiated into fibroblasts, chondrocytes, and osteoblasts and that they develop into fibrous tissue, cartilage, or bone, as a result of mechanical stimulation. In this study, we developed a bioreactor system, which is composed of a reactor vessel that provides the required cell culture environment, an environment controlling chamber to control the media, a gas mixer, and a reactor motion control subsystem to apply mechanical stimuli to the cells. For the MSC culture, We used a poly-urethane (PU) scaffold, with a collagen coating to ensure improved cohesion ratio. Then, we transferred the cultivated MSCs in the PU scaffold, cultured the cells in the bioreactor system, and confirmed the proliferation, differentiation, and ossification processes, resulting from mechanical stimuli.

KW - Bioreactor

KW - Mechanical stimulation

KW - Mesenchymal stem cell

KW - Polyurethane scaffold

UR - http://www.scopus.com/inward/record.url?scp=77953837829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953837829&partnerID=8YFLogxK

U2 - 10.3795/KSME-A.2010.34.6.675

DO - 10.3795/KSME-A.2010.34.6.675

M3 - Article

AN - SCOPUS:77953837829

VL - 34

SP - 675

EP - 681

JO - Transactions of the Korean Society of Mechanical Engineers, A

JF - Transactions of the Korean Society of Mechanical Engineers, A

SN - 1226-4873

IS - 6

ER -