Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

Atsushi Yagishita, Tasuku Ueno, Hiroyasu Esumi, Hideyuki Saya, Kazuhiro Kaneko, Katsuya Tsuchihara, Yasuteru Urano

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

Original languageEnglish
Pages (from-to)302-306
Number of pages5
JournalBioconjugate Chemistry
Volume28
Issue number2
DOIs
Publication statusPublished - 2017 Feb 15

Fingerprint

Aldehydes
Fluorescent Dyes
Cells
Aldehyde Dehydrogenase
Fluorescence microscopy
Stem cells
Visualization
Neoplastic Stem Cells
Fluorescence Microscopy
Epithelial Cells
Cell Line
Oxidoreductases
bovine corneal protein 54

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells. / Yagishita, Atsushi; Ueno, Tasuku; Esumi, Hiroyasu; Saya, Hideyuki; Kaneko, Kazuhiro; Tsuchihara, Katsuya; Urano, Yasuteru.

In: Bioconjugate Chemistry, Vol. 28, No. 2, 15.02.2017, p. 302-306.

Research output: Contribution to journalArticle

Yagishita, Atsushi ; Ueno, Tasuku ; Esumi, Hiroyasu ; Saya, Hideyuki ; Kaneko, Kazuhiro ; Tsuchihara, Katsuya ; Urano, Yasuteru. / Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells. In: Bioconjugate Chemistry. 2017 ; Vol. 28, No. 2. pp. 302-306.
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