Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

Atsushi Yagishita, Tasuku Ueno, Hiroyasu Esumi, Hideyuki Saya, Kazuhiro Kaneko, Katsuya Tsuchihara, Yasuteru Urano

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

Original languageEnglish
Pages (from-to)302-306
Number of pages5
JournalBioconjugate Chemistry
Volume28
Issue number2
DOIs
Publication statusPublished - 2017 Feb 15

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells'. Together they form a unique fingerprint.

  • Cite this