Development of in situ zymography to localize active matrix metalloproteinase-7 (matrilysin-1)

Ryoichi Nemori, Masayoshi Yamamoto, Fumio Kataoka, Gakuji Hashimoto, Hiroshi Arakatsu, Takayuki Shiomi, Yasunori Okada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Matrix metalloproteinase-7 (MMP-7) is upregulated during carcinogenesis and its expression correlates with metastasis of human endometrial and gastrointestinal carcinomas. In the present study, we have developed a new method to localize the activity of MMP-7 within tissues. Polyethylene terephthalate films were uniformly coated with crosslinked carboxymethylated transferrin (CCm-Tf) as a substrate and incubated with frozen tissue sections mounted on the films. CCm-Tf on the films was degraded selectively by MMP-7, but showed little or no susceptibility to MMP-1, -2, -3, -9, or -13; MT1-MMP; MT3-MMP; or ADAMTS4. Although some serine proteinases such as elastase also digested CCm-Tf, CCm-Tf films impregnated with serine proteinase inhibitors prevented the digestion. When frozen sections of human endometrial carcinoma and lung carcinoma tissues were incubated on CCm-Tf films or those treated with proteinase inhibitors, the activity was detected in the carcinoma cell nests, where MMP-7 was immunolocalized. The present in situ zymography using CCm-Tf may be a useful method to analyze the functions of MMP-7 in pathophysiological conditions.

Original languageEnglish
Pages (from-to)1227-1234
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume53
Issue number10
DOIs
Publication statusPublished - 2005 Oct 1

Keywords

  • Carcinoma invasion
  • In situ zymography
  • Matrilysin-1
  • Matrix metalloproteinase-7
  • Proteolytic activity
  • Tissue localization

ASJC Scopus subject areas

  • Anatomy
  • Histology

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    Nemori, R., Yamamoto, M., Kataoka, F., Hashimoto, G., Arakatsu, H., Shiomi, T., & Okada, Y. (2005). Development of in situ zymography to localize active matrix metalloproteinase-7 (matrilysin-1). Journal of Histochemistry and Cytochemistry, 53(10), 1227-1234. https://doi.org/10.1369/jhc.5A6631.2005