Development of measurement and assessment technology for evaluation of bone regeneration

Noriko Kotobuki, Motohiro Hirose, Koji Ioku, Akira Sakaguchi, Akifumi Iwarna, Masaki Harada, Hiroshi Yamamoto, Hajime Ohgushi

Research output: Contribution to journalArticle

Abstract

Since 2001, we have clinically utilized human bone marrow-derived mesenchymal stem cells (MSCs) for bone regeneration. The osteogenic ability of MSCs has been assessed by measurement of alkaline phosphatase activity and calcium deposition. As for the detection of in vitro calcium deposition, we have established the method using calcein, which is a calcium-binding fluorescence material. Using this fluorescence material, we could observe the calcium deposition and then estimate the value of calcium deposition. In this report, we cultured rat MSCs on culture plate as well as transparent β-TCP, and calcium deposition was visualized and quantitated using an image analyzer. After 2 weeks differentiation of rat MSCs to osteoblasts, calcium deposition on β-TCP was observed as a signal of calcium-binding fluorescence. This fluorescence signal was also quantitated with an image analyzer.

Original languageEnglish
Pages (from-to)997-1000
Number of pages4
JournalKey Engineering Materials
Volume361-363 II
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Calcium
Bone
Stem cells
Fluorescence
Rats
Osteoblasts
Phosphatases
Cell culture
Alkaline Phosphatase

Keywords

  • Bone regeneration
  • Calcium-binding fluorescence
  • Mesenchymal stem cells (MSCs)
  • Transparent β-TCP

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemical Engineering (miscellaneous)

Cite this

Kotobuki, N., Hirose, M., Ioku, K., Sakaguchi, A., Iwarna, A., Harada, M., ... Ohgushi, H. (2008). Development of measurement and assessment technology for evaluation of bone regeneration. Key Engineering Materials, 361-363 II, 997-1000.

Development of measurement and assessment technology for evaluation of bone regeneration. / Kotobuki, Noriko; Hirose, Motohiro; Ioku, Koji; Sakaguchi, Akira; Iwarna, Akifumi; Harada, Masaki; Yamamoto, Hiroshi; Ohgushi, Hajime.

In: Key Engineering Materials, Vol. 361-363 II, 2008, p. 997-1000.

Research output: Contribution to journalArticle

Kotobuki, N, Hirose, M, Ioku, K, Sakaguchi, A, Iwarna, A, Harada, M, Yamamoto, H & Ohgushi, H 2008, 'Development of measurement and assessment technology for evaluation of bone regeneration', Key Engineering Materials, vol. 361-363 II, pp. 997-1000.
Kotobuki N, Hirose M, Ioku K, Sakaguchi A, Iwarna A, Harada M et al. Development of measurement and assessment technology for evaluation of bone regeneration. Key Engineering Materials. 2008;361-363 II:997-1000.
Kotobuki, Noriko ; Hirose, Motohiro ; Ioku, Koji ; Sakaguchi, Akira ; Iwarna, Akifumi ; Harada, Masaki ; Yamamoto, Hiroshi ; Ohgushi, Hajime. / Development of measurement and assessment technology for evaluation of bone regeneration. In: Key Engineering Materials. 2008 ; Vol. 361-363 II. pp. 997-1000.
@article{c87ac74f8a15463a8644cfe6ddb9ca19,
title = "Development of measurement and assessment technology for evaluation of bone regeneration",
abstract = "Since 2001, we have clinically utilized human bone marrow-derived mesenchymal stem cells (MSCs) for bone regeneration. The osteogenic ability of MSCs has been assessed by measurement of alkaline phosphatase activity and calcium deposition. As for the detection of in vitro calcium deposition, we have established the method using calcein, which is a calcium-binding fluorescence material. Using this fluorescence material, we could observe the calcium deposition and then estimate the value of calcium deposition. In this report, we cultured rat MSCs on culture plate as well as transparent β-TCP, and calcium deposition was visualized and quantitated using an image analyzer. After 2 weeks differentiation of rat MSCs to osteoblasts, calcium deposition on β-TCP was observed as a signal of calcium-binding fluorescence. This fluorescence signal was also quantitated with an image analyzer.",
keywords = "Bone regeneration, Calcium-binding fluorescence, Mesenchymal stem cells (MSCs), Transparent β-TCP",
author = "Noriko Kotobuki and Motohiro Hirose and Koji Ioku and Akira Sakaguchi and Akifumi Iwarna and Masaki Harada and Hiroshi Yamamoto and Hajime Ohgushi",
year = "2008",
language = "English",
volume = "361-363 II",
pages = "997--1000",
journal = "Key Engineering Materials",
issn = "1013-9826",
publisher = "Trans Tech Publications",

}

TY - JOUR

T1 - Development of measurement and assessment technology for evaluation of bone regeneration

AU - Kotobuki, Noriko

AU - Hirose, Motohiro

AU - Ioku, Koji

AU - Sakaguchi, Akira

AU - Iwarna, Akifumi

AU - Harada, Masaki

AU - Yamamoto, Hiroshi

AU - Ohgushi, Hajime

PY - 2008

Y1 - 2008

N2 - Since 2001, we have clinically utilized human bone marrow-derived mesenchymal stem cells (MSCs) for bone regeneration. The osteogenic ability of MSCs has been assessed by measurement of alkaline phosphatase activity and calcium deposition. As for the detection of in vitro calcium deposition, we have established the method using calcein, which is a calcium-binding fluorescence material. Using this fluorescence material, we could observe the calcium deposition and then estimate the value of calcium deposition. In this report, we cultured rat MSCs on culture plate as well as transparent β-TCP, and calcium deposition was visualized and quantitated using an image analyzer. After 2 weeks differentiation of rat MSCs to osteoblasts, calcium deposition on β-TCP was observed as a signal of calcium-binding fluorescence. This fluorescence signal was also quantitated with an image analyzer.

AB - Since 2001, we have clinically utilized human bone marrow-derived mesenchymal stem cells (MSCs) for bone regeneration. The osteogenic ability of MSCs has been assessed by measurement of alkaline phosphatase activity and calcium deposition. As for the detection of in vitro calcium deposition, we have established the method using calcein, which is a calcium-binding fluorescence material. Using this fluorescence material, we could observe the calcium deposition and then estimate the value of calcium deposition. In this report, we cultured rat MSCs on culture plate as well as transparent β-TCP, and calcium deposition was visualized and quantitated using an image analyzer. After 2 weeks differentiation of rat MSCs to osteoblasts, calcium deposition on β-TCP was observed as a signal of calcium-binding fluorescence. This fluorescence signal was also quantitated with an image analyzer.

KW - Bone regeneration

KW - Calcium-binding fluorescence

KW - Mesenchymal stem cells (MSCs)

KW - Transparent β-TCP

UR - http://www.scopus.com/inward/record.url?scp=38149000233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38149000233&partnerID=8YFLogxK

M3 - Article

VL - 361-363 II

SP - 997

EP - 1000

JO - Key Engineering Materials

JF - Key Engineering Materials

SN - 1013-9826

ER -