Development of Melanocyte Progenitors in Murine Steel Mutant Neural Crest Explants Cultured with Stem Cell Factor, Endothelin-3, or TPA

Hirotake Ono, Yoko Kawa, Mari Asano, Masaru Ito, Atsuko Takano, Yasuo Kubota, Jiro Matsumoto, Masako Mizoguchi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Stem cell factor (SCF) has been suggested to be indispensable for the development of neural crest cells into melanocytes because Steel mutant mice (i.e., Sl/lSld) have no pigmented hairs. On the other hand, it has been demonstrated that the addition of endothelin 3 (ET-3) or TPA to neural crest cell cultures can induce melanocyte differentiation with-out addition of extrinsic SCF. In this study, we excluded the influence of intrinsic SCF by using Sl/Sl mouse embryos to study more precisely the effects of natural cytokines, such as extrinsic soluble SCF or ET-3, or chemical reagents, such as TPA or cholera toxin. We found that SCF is supplied within the wild-type neural crest explants and that ET-3 cannot induce melanocyte differentiation or proliferation without SCF. These results indicate that SCF plays a critical role in survival or G1/S entry of melanocyte progenitors and that SCF initially stimulates their proliferation and then ET-3 accelerates their proliferation and differentiation. TPA has the ability to elicit neural crest cell differentiation into melanocytes without exogenously added SCF but it is not as effective as SCF because many more melanocytes developed in the wild-type neural crest explants cultured with TPA.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalPigment Cell Research
Volume11
Issue number5
DOIs
Publication statusPublished - 1998 Oct

Keywords

  • ACK2
  • Lethal spotting
  • Melanoblast
  • Neural crest
  • Phorbol ester
  • Piebald
  • c-kit

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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