Development of mesenchymal stem cells partially originate from the neural crest

Satoru Morikawa, Yo Mabuchi, Kunimichi Niibe, Sadafumi Suzuki, Narihito Nagoshi, Takehiko Sunabori, Shigeto Shimmura, Yasuo Nagai, Taneaki Nakagawa, Hideyuki Okano, Yumi Matsuzaki

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as ''dedifferentiation'' or ''transdifferentiation''. However, since MSCs have only been defined in vitro, much of their development in vivo is still unknown. Here, we prospectively identified MSCs in the bone marrow from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. EGFP-positive MSCs formed spheres that expressed neural crest stem cell genes and differentiated into neurons, glial cells, and myofibroblasts. Interestingly, we observed MSCs both in the GFP+ and GFP- fraction and found that there were no significant differences in the in vitro characteristics between these two populations. Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest.

Original languageEnglish
Pages (from-to)1114-1119
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume379
Issue number4
DOIs
Publication statusPublished - 2009 Feb 20

Fingerprint

Neural Crest
Stem cells
Mesenchymal Stromal Cells
Bone Marrow
Bone
Myofibroblasts
Neural Stem Cells
Stromal Cells
Neuroglia
Transgenic Mice
Stem Cells
Neurons
Genes
Tissue
Population

Keywords

  • Developmental origin
  • Flow cytometry
  • Mesenchymal stem cells
  • Neural crest
  • Prospective isolation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Development of mesenchymal stem cells partially originate from the neural crest. / Morikawa, Satoru; Mabuchi, Yo; Niibe, Kunimichi; Suzuki, Sadafumi; Nagoshi, Narihito; Sunabori, Takehiko; Shimmura, Shigeto; Nagai, Yasuo; Nakagawa, Taneaki; Okano, Hideyuki; Matsuzaki, Yumi.

In: Biochemical and Biophysical Research Communications, Vol. 379, No. 4, 20.02.2009, p. 1114-1119.

Research output: Contribution to journalArticle

Morikawa, Satoru ; Mabuchi, Yo ; Niibe, Kunimichi ; Suzuki, Sadafumi ; Nagoshi, Narihito ; Sunabori, Takehiko ; Shimmura, Shigeto ; Nagai, Yasuo ; Nakagawa, Taneaki ; Okano, Hideyuki ; Matsuzaki, Yumi. / Development of mesenchymal stem cells partially originate from the neural crest. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 379, No. 4. pp. 1114-1119.
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