TY - JOUR
T1 - Development of microsatellite markers for the Japanese giant salamander (Andrias japonicus) through next-generation sequencing, and cross-amplification in its congener
AU - Yoshikawa, Natsuhiko
AU - Matsui, Masafumi
AU - Hayano, Azusa
AU - Inoue-Murayama, Miho
N1 - Funding Information:
Acknowledgments The Japan Agency for Cultural Affairs and educatory committees of relevant local governments granted permission to study protected Japanese giant salamander. We thank Y. Hattori, H. Onuma and K. Nishikawa for help in collecting specimens. We are also grateful to Kawakami-Dam Project Office of Japan Water Agency and curators of aquariums and zoos for providing valuable specimens. This work was partly supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Japan, through the Japan Society for the Promotion of Science to MM (Nos. 20510215, 23510215), and from Conservation International to NY.
PY - 2012/12
Y1 - 2012/12
N2 - A giant salamander Andrias japonicus, endemic to western Japan is ranked as near threatened. However, genetic markers necessary for estimating genetic diversity and structures for conservation measures have been limited. Therefore, we developed seventeen novel microsatellite markers from A. japonicus, and tried cross-amplification in its congener, Andrias davidianus. The number of alleles and expected heterozygosity at each locus were 1-5 (mean = 2. 53) and 0-0. 66, respectively, in A. japonicus. Cross-amplification in A. davidianus succeeded in 15 of 17 loci. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of A. japonicus and A. davidianus.
AB - A giant salamander Andrias japonicus, endemic to western Japan is ranked as near threatened. However, genetic markers necessary for estimating genetic diversity and structures for conservation measures have been limited. Therefore, we developed seventeen novel microsatellite markers from A. japonicus, and tried cross-amplification in its congener, Andrias davidianus. The number of alleles and expected heterozygosity at each locus were 1-5 (mean = 2. 53) and 0-0. 66, respectively, in A. japonicus. Cross-amplification in A. davidianus succeeded in 15 of 17 loci. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of A. japonicus and A. davidianus.
KW - Andrias davidianus
KW - Andrias japonicus
KW - Conservation
KW - Genetic diversity
KW - Microsatellite
KW - Next-generation sequencing
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U2 - 10.1007/s12686-012-9685-7
DO - 10.1007/s12686-012-9685-7
M3 - Article
AN - SCOPUS:84867590289
SN - 1877-7252
VL - 4
SP - 971
EP - 974
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 4
ER -