Development of microsatellite markers for the Japanese giant salamander (Andrias japonicus) through next-generation sequencing, and cross-amplification in its congener

Natsuhiko Yoshikawa, Masafumi Matsui, Azusa Hayano, Miho Inoue-Murayama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A giant salamander Andrias japonicus, endemic to western Japan is ranked as near threatened. However, genetic markers necessary for estimating genetic diversity and structures for conservation measures have been limited. Therefore, we developed seventeen novel microsatellite markers from A. japonicus, and tried cross-amplification in its congener, Andrias davidianus. The number of alleles and expected heterozygosity at each locus were 1-5 (mean = 2. 53) and 0-0. 66, respectively, in A. japonicus. Cross-amplification in A. davidianus succeeded in 15 of 17 loci. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of A. japonicus and A. davidianus.

Original languageEnglish
Pages (from-to)971-974
Number of pages4
JournalConservation Genetics Resources
Volume4
Issue number4
DOIs
Publication statusPublished - 2012 Dec 1
Externally publishedYes

Fingerprint

Urodela
Genetic Structures
salamanders and newts
Microsatellite Repeats
genetic structure
amplification
microsatellite repeats
genetic variation
loci
genetic marker
conservation management
Genetic Markers
heterozygosity
allele
Japan
planning
Alleles
alleles
genetic markers
Andrias

Keywords

  • Andrias davidianus
  • Andrias japonicus
  • Conservation
  • Genetic diversity
  • Microsatellite
  • Next-generation sequencing

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Development of microsatellite markers for the Japanese giant salamander (Andrias japonicus) through next-generation sequencing, and cross-amplification in its congener. / Yoshikawa, Natsuhiko; Matsui, Masafumi; Hayano, Azusa; Inoue-Murayama, Miho.

In: Conservation Genetics Resources, Vol. 4, No. 4, 01.12.2012, p. 971-974.

Research output: Contribution to journalArticle

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