Development of microsatellite markers for the Japanese giant salamander (Andrias japonicus) through next-generation sequencing, and cross-amplification in its congener

Natsuhiko Yoshikawa, Masafumi Matsui, Azusa Hayano, Miho Inoue-Murayama

Research output: Contribution to journalArticle

4 Citations (Scopus)


A giant salamander Andrias japonicus, endemic to western Japan is ranked as near threatened. However, genetic markers necessary for estimating genetic diversity and structures for conservation measures have been limited. Therefore, we developed seventeen novel microsatellite markers from A. japonicus, and tried cross-amplification in its congener, Andrias davidianus. The number of alleles and expected heterozygosity at each locus were 1-5 (mean = 2. 53) and 0-0. 66, respectively, in A. japonicus. Cross-amplification in A. davidianus succeeded in 15 of 17 loci. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of A. japonicus and A. davidianus.

Original languageEnglish
Pages (from-to)971-974
Number of pages4
JournalConservation Genetics Resources
Issue number4
Publication statusPublished - 2012 Dec 1
Externally publishedYes



  • Andrias davidianus
  • Andrias japonicus
  • Conservation
  • Genetic diversity
  • Microsatellite
  • Next-generation sequencing

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this