TY - JOUR
T1 - Development of neuraminidase detection using gold nanoparticles boron-doped diamond electrodes
AU - Wahyuni, Wulan T.
AU - Ivandini, Tribidasari A.
AU - Saepudin, Endang
AU - Einaga, Yasuaki
PY - 2016/3/15
Y1 - 2016/3/15
N2 - Gold nanoparticles-modified boron-doped diamond (AuNPs-BDD) electrodes, which were prepared with a self-assembly deposition of AuNPs at amine-terminated boron-doped diamond, were examined for voltammetric detection of neuraminidase (NA). The detection method was performed based on the difference of electrochemical responses of zanamivir at gold surface before and after the reaction with NA in phosphate buffer solution (PBS, pH 5.5). A linear calibration curve for zanamivir in 0.1 M PBS in the absence of NA was achieved in the concentration range of 1 × 10-6 to 1 × 10-5 M (R2 = 0.99) with an estimated limit of detection (LOD) of 2.29 × 10-6 M. Furthermore, using its reaction with 1.00 × 10-5 M zanamivir, a linear calibration curve of NA can be obtained in the concentration range of 0-12 mU (R2 = 0.99) with an estimated LOD of 0.12 mU. High reproducibility was shown with a relative standard deviation (RSD) of 1.14% (n = 30). These performances could be maintained when the detection was performed in mucin matrix. Comparison performed using gold-modified BDD (Au-BDD) electrodes suggested that the good performance of the detection method is due to the stability of the gold particles position at the BDD surface.
AB - Gold nanoparticles-modified boron-doped diamond (AuNPs-BDD) electrodes, which were prepared with a self-assembly deposition of AuNPs at amine-terminated boron-doped diamond, were examined for voltammetric detection of neuraminidase (NA). The detection method was performed based on the difference of electrochemical responses of zanamivir at gold surface before and after the reaction with NA in phosphate buffer solution (PBS, pH 5.5). A linear calibration curve for zanamivir in 0.1 M PBS in the absence of NA was achieved in the concentration range of 1 × 10-6 to 1 × 10-5 M (R2 = 0.99) with an estimated limit of detection (LOD) of 2.29 × 10-6 M. Furthermore, using its reaction with 1.00 × 10-5 M zanamivir, a linear calibration curve of NA can be obtained in the concentration range of 0-12 mU (R2 = 0.99) with an estimated LOD of 0.12 mU. High reproducibility was shown with a relative standard deviation (RSD) of 1.14% (n = 30). These performances could be maintained when the detection was performed in mucin matrix. Comparison performed using gold-modified BDD (Au-BDD) electrodes suggested that the good performance of the detection method is due to the stability of the gold particles position at the BDD surface.
KW - Electroanalysis
KW - Enzymatic inhibition
KW - Gold nanoparticle
KW - Neuraminidase
KW - Zanamivir
UR - http://www.scopus.com/inward/record.url?scp=84969389443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84969389443&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2015.12.003
DO - 10.1016/j.ab.2015.12.003
M3 - Article
C2 - 26717895
AN - SCOPUS:84969389443
VL - 497
SP - 68
EP - 75
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
ER -