Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging

Yuichiro Koide, Yasuteru Urano, Kenjiro Hanaoka, Wen Piao, Moriaki Kusakabe, Nae Saito, Takuya Terai, Takayoshi Okabe, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

192 Citations (Scopus)

Abstract

We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si-rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes.

Original languageEnglish
Pages (from-to)5029-5031
Number of pages3
JournalJournal of the American Chemical Society
Volume134
Issue number11
DOIs
Publication statusPublished - 2012 Mar 21
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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