Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain

Wei Ran Tang, Nobutaka Kiyokawa, Tomoko Eguchi, Jun Matsui, Hisami Takenouchi, Daisuk Honma, Hiroshi Yasue, Shin Enosawa, Kenichi Mimori, Mitsuko Itagaki, Tomoko Taguchi, Yohko U. Katagiri, Hajime Okita, Hiroshi Amemiya, Junichiro Fujimoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I α chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immunohistochemistry using SLA class I α chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I α chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.

Original languageEnglish
Pages (from-to)187-191
Number of pages5
JournalHybridoma and Hybridomics
Volume23
Issue number3
DOIs
Publication statusPublished - 2004 Jun 1
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Genetics

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    Tang, W. R., Kiyokawa, N., Eguchi, T., Matsui, J., Takenouchi, H., Honma, D., Yasue, H., Enosawa, S., Mimori, K., Itagaki, M., Taguchi, T., Katagiri, Y. U., Okita, H., Amemiya, H., & Fujimoto, J. (2004). Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I α chain. Hybridoma and Hybridomics, 23(3), 187-191. https://doi.org/10.1089/1536859041224253