TY - JOUR
T1 - Development of photo-controllable hydrogen sulfide donor applicable in live cells
AU - Fukushima, Naoki
AU - Ieda, Naoya
AU - Kawaguchi, Mitsuyasu
AU - Sasakura, Kiyoshi
AU - Nagano, Tetsuo
AU - Hanaoka, Kenjiro
AU - Miyata, Naoki
AU - Nakagawa, Hidehiko
N1 - Funding Information:
This work was supported by the JST PRESTO program (H.N.) of the Japan Science and Technology Agency , as well as by a JSPS KAKENHI Grant Number 25293028 (H.N.), a Grant from Hoansha Foundation (H.N.), and a Sasakawa Scientific Research Grant from The Japan Scientific Society (No. 25-311 , N.F.).
Publisher Copyright:
© 2014 The Authors. Published by Elsevier Ltd.
PY - 2015/1/15
Y1 - 2015/1/15
N2 - Hydrogen sulfide (H2S) has multiple physiological roles, for example, in vasodilation and inflammation. It is a highly reactive gas under ambient conditions, so controllable H2S donors are required for studying its biological functions. Here, we describe the design, synthesis and application of a H2S donor (SPD-2) that utilizes xanthone photochemistry to control H2S release. H2S generation from SPD-2 was completely dependent on UVA-irradiation (325-385 nm), as confirmed by methylene blue assay and by the use of a H2S-selective fluorescent probe. SPD-2 was confirmed to provide controlled H2S delivery in live cells, and should be suitable for various biological applications.
AB - Hydrogen sulfide (H2S) has multiple physiological roles, for example, in vasodilation and inflammation. It is a highly reactive gas under ambient conditions, so controllable H2S donors are required for studying its biological functions. Here, we describe the design, synthesis and application of a H2S donor (SPD-2) that utilizes xanthone photochemistry to control H2S release. H2S generation from SPD-2 was completely dependent on UVA-irradiation (325-385 nm), as confirmed by methylene blue assay and by the use of a H2S-selective fluorescent probe. SPD-2 was confirmed to provide controlled H2S delivery in live cells, and should be suitable for various biological applications.
KW - Caged HS
KW - Hydrogen sulfide
KW - Photolabile protecting group
KW - Xanthone photocage
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U2 - 10.1016/j.bmcl.2014.11.084
DO - 10.1016/j.bmcl.2014.11.084
M3 - Article
C2 - 25499432
AN - SCOPUS:84922258158
SN - 0960-894X
VL - 25
SP - 175
EP - 178
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 2
ER -