Development of quantitative method for determination of γ-glutamyl peptides by capillary electrophoresis tandem mass spectrometry: An efficient approach avoiding matrix effect

Akiyoshi Hirayama, Kaori Igarashi, Masaru Tomita, Tomoyoshi Soga

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Serum γ-glutamyl di- and tripeptides have proven to be useful biomarkers to accurately predict nine different forms of liver disease. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with multiple reaction monitoring (MRM), serum and liver samples spiked with γ-glutamyl peptide standards were analyzed to estimate accuracy. Unexpectedly, the recovery rates for several γ-glutamyl peptides in the serum samples were quite low, whereas values for some γ-glutamyl peptides in the liver samples were highly elevated. Most of these peptides were barely retained on the reverse-phase column, resulting in significant ion suppression or enhancement. In contrast, a capillary electrophoresis tandem mass spectrometry (CE-MS/MS) method with MRM was minimally affected by matrix effects. Of the 39 tested compounds, most of γ-glutamyl peptides that did not contain a thiol substituent in its structure gave acceptable recoveries (70-120%), and limits of detection for the analytes were between 3.6 and 800. nmol/l with pressure injection at 5. kPa for 10. s (ca. 10. nl). The CE-MS/MS method provided high resolution and proved to be highly selective and sensitive, its utility being demonstrated by the determination of γ-glutamyl di- and tripeptides in serum and liver samples.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalJournal of Chromatography A
Volume1369
DOIs
Publication statusPublished - 2014 Nov 21

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Tandem Mass Spectrometry
Mass spectrometry
Liver
Peptides
Dipeptides
Serum
Recovery
Monitoring
Liquid chromatography
Biomarkers
Sulfhydryl Compounds
Liquid Chromatography
Limit of Detection
Liver Diseases
Ions
Pressure
Injections

Keywords

  • Capillary electrophoresis tandem mass spectrometry (CE-MS/MS)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
  • Liver disease
  • Matrix effect
  • γ-Glutamyl peptides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

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abstract = "Serum γ-glutamyl di- and tripeptides have proven to be useful biomarkers to accurately predict nine different forms of liver disease. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with multiple reaction monitoring (MRM), serum and liver samples spiked with γ-glutamyl peptide standards were analyzed to estimate accuracy. Unexpectedly, the recovery rates for several γ-glutamyl peptides in the serum samples were quite low, whereas values for some γ-glutamyl peptides in the liver samples were highly elevated. Most of these peptides were barely retained on the reverse-phase column, resulting in significant ion suppression or enhancement. In contrast, a capillary electrophoresis tandem mass spectrometry (CE-MS/MS) method with MRM was minimally affected by matrix effects. Of the 39 tested compounds, most of γ-glutamyl peptides that did not contain a thiol substituent in its structure gave acceptable recoveries (70-120{\%}), and limits of detection for the analytes were between 3.6 and 800. nmol/l with pressure injection at 5. kPa for 10. s (ca. 10. nl). The CE-MS/MS method provided high resolution and proved to be highly selective and sensitive, its utility being demonstrated by the determination of γ-glutamyl di- and tripeptides in serum and liver samples.",
keywords = "Capillary electrophoresis tandem mass spectrometry (CE-MS/MS), Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Liver disease, Matrix effect, γ-Glutamyl peptides",
author = "Akiyoshi Hirayama and Kaori Igarashi and Masaru Tomita and Tomoyoshi Soga",
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AU - Hirayama, Akiyoshi

AU - Igarashi, Kaori

AU - Tomita, Masaru

AU - Soga, Tomoyoshi

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