Development of tissue-targeting hemagglutinating virus of Japan envelope vector for successful delivery of therapeutic gene to mouse skin

Masako Kawachi, Katsuto Tamai, Kotaro Saga, Takehiko Yamazaki, Hiroshi Fujita, Takashi Shimbo, Yasushi Kikuchi, Keisuke Nimura, Koji Nishifuji, Masayuki Amagai, Jouni Uitto, Yasufumi Kaneda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We report a novel strategy for constructing a tissue-targeting hemagglutinating virus of Japan (HVJ; Sendai virus) envelope vector (HVJ-E), and its application in gene therapy of a mouse model of genetic skin disease. Chimeric genes encoding viral F protein and green fluorescent protein (GFP) were constructed on the basis of various deletion mutants. The product of one chimeric gene, containing signal peptide, transmembrane domain, and the cytoplasmic tail of F protein, was transported to the cell surface and incorporated into new viruses released from HVJ-infected LLC-MK2 cells. For tissue targeting, in the preceding construct GFP was replaced with single-chain antibody (scFv) against mouse desmoglein 3 (mDsg3), a desmosomal cadherin found in basal layer keratinocytes of the skin. HVJ encoding scFv-F chimeric protein bound to mDsg3-coated plates much more efficiently than did wild-type HVJ. When chimeric HVJ was injected into a skin blister of a mouse model of epidermolysis bullosa, in which defective expression of type VII collagen results in a failure to secure epidermis to the underlying dermis, viral F protein expression was detected in most of the basal keratinocytes. Furthermore, chimeric HVJ-E introduced type VII collagen expression more efficiently compared with wild-type HVJ in basal keratinocytes of type VII collagen-deficient mouse skin, resulting in efficient amelioration of the genetic defect. Thus, a novel tissue-targeting HVJ-E could be used to successfully target epidermal keratinocytes both in vitro and in vivo.

Original languageEnglish
Pages (from-to)881-894
Number of pages14
JournalHuman Gene Therapy
Volume18
Issue number10
DOIs
Publication statusPublished - 2007 Oct 1

Fingerprint

Sendai virus
Collagen Type VII
Keratinocytes
Desmoglein 3
Skin
Genes
Viral Proteins
Green Fluorescent Proteins
Genetic Skin Diseases
Desmosomal Cadherins
Epidermolysis Bullosa
Therapeutics
Single-Chain Antibodies
Blister
Dermis
Protein Sorting Signals
Epidermis
Genetic Therapy
Tail
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Development of tissue-targeting hemagglutinating virus of Japan envelope vector for successful delivery of therapeutic gene to mouse skin. / Kawachi, Masako; Tamai, Katsuto; Saga, Kotaro; Yamazaki, Takehiko; Fujita, Hiroshi; Shimbo, Takashi; Kikuchi, Yasushi; Nimura, Keisuke; Nishifuji, Koji; Amagai, Masayuki; Uitto, Jouni; Kaneda, Yasufumi.

In: Human Gene Therapy, Vol. 18, No. 10, 01.10.2007, p. 881-894.

Research output: Contribution to journalArticle

Kawachi, M, Tamai, K, Saga, K, Yamazaki, T, Fujita, H, Shimbo, T, Kikuchi, Y, Nimura, K, Nishifuji, K, Amagai, M, Uitto, J & Kaneda, Y 2007, 'Development of tissue-targeting hemagglutinating virus of Japan envelope vector for successful delivery of therapeutic gene to mouse skin', Human Gene Therapy, vol. 18, no. 10, pp. 881-894. https://doi.org/10.1089/hum.2007.046
Kawachi, Masako ; Tamai, Katsuto ; Saga, Kotaro ; Yamazaki, Takehiko ; Fujita, Hiroshi ; Shimbo, Takashi ; Kikuchi, Yasushi ; Nimura, Keisuke ; Nishifuji, Koji ; Amagai, Masayuki ; Uitto, Jouni ; Kaneda, Yasufumi. / Development of tissue-targeting hemagglutinating virus of Japan envelope vector for successful delivery of therapeutic gene to mouse skin. In: Human Gene Therapy. 2007 ; Vol. 18, No. 10. pp. 881-894.
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