Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation

Tetsuya Mizuno, Keitaro Umezawa, Yutaka Shindo, Daniel Citterio, Kotaro Oka, Koji Suzuki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca2+/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca2+ indicator) reveals its usefulness as a new bioimaging tool.

Original languageEnglish
Pages (from-to)1007-1018
Number of pages12
JournalJournal of Fluorescence
Volume23
Issue number5
DOIs
Publication statusPublished - 2013 Sep

Fingerprint

Fura-2
dyad
Labels
Albumins
Esters
Color
Fluorescence
Infrared radiation
Wavelength
Fluorophores
Biomolecules
Labeling
Imaging techniques
pyrene

Keywords

  • Bioimaging
  • BODIPY
  • Fluorescent dyads
  • Near-infrared fluorophore

ASJC Scopus subject areas

  • Spectroscopy
  • Biochemistry
  • Clinical Biochemistry

Cite this

Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation. / Mizuno, Tetsuya; Umezawa, Keitaro; Shindo, Yutaka; Citterio, Daniel; Oka, Kotaro; Suzuki, Koji.

In: Journal of Fluorescence, Vol. 23, No. 5, 09.2013, p. 1007-1018.

Research output: Contribution to journalArticle

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