Developmental and hormonal regulation of murine scavenger receptor, class B, type 1

Guoqing Cao, Liping Zhao, Herbert Stangl, Tomonobu Hasegawa, James A. Richardson, Keith L. Parker, Helen H. Hobbs

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The scavenger receptor, class B, type I (SR-BI), is the predominant receptor that supplies plasma cholesterol to steroidogenic tissues in rodents. We showed previously that steroidogenic factor-1 (SF-1) binds a sequence in the human SR-BI promoter whose integrity is required for high-level SR-BI expression in cultured adrenocortical tumor cells. We now provide in vivo evidence that SF-1 regulates SR-BI. During mouse embryogenesis, SR-BI mRNA was initially expressed in the genital ridge of both sexes and persisted in the developing testes but not ovary. This sexually dimorphic expression profile of SR-BI expression in the gonads mirrors that of SF-1. No SR-BI mRNA was detected in the gonadal ridge of day 11.5 SF-1 knockout embryos. Both SR-BI and SF-1 mRNA were expressed in the cortical cells of the nascent adrenal glands. These studies directly support SF-1 participating in the regulation of SR-BI in vivo. We examined the effect of cAMP on SR-BI mRNA and protein in mouse adrenocortical (Y1-BS1) and testicular carcinoma Leydig (MA-10) cells. The time courses of induction were strikingly similar to those described for other cAMP- and SF-1-regulated genes. Addition of lipoproteins reduced SR-BI expression in Y1-BS1 cells, an effect that was reversed by administration of cAMP analogs. SR-BI mRNA and protein were expressed at high levels in the adrenal glands of knockout mice lacking the steroidogenic acute regulatory protein; these mice have extensive lipid deposits in the adrenocortical cells and high circulating levels of ACTH. Taken together, these studies suggest that trophic hormones can override the suppressive effect of cholesterol on SR-BI expression, thus ensuring that steroidogenesis is maintained during stress.

Original languageEnglish
Pages (from-to)1460-1473
Number of pages14
JournalMolecular Endocrinology
Volume13
Issue number9
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

CD36 Antigens
Steroidogenic Factor 1
Messenger RNA
Adrenal Glands
Cholesterol
Cultured Tumor Cells
Gonads
Knockout Mice
Adrenocorticotropic Hormone

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Cao, G., Zhao, L., Stangl, H., Hasegawa, T., Richardson, J. A., Parker, K. L., & Hobbs, H. H. (1999). Developmental and hormonal regulation of murine scavenger receptor, class B, type 1. Molecular Endocrinology, 13(9), 1460-1473.

Developmental and hormonal regulation of murine scavenger receptor, class B, type 1. / Cao, Guoqing; Zhao, Liping; Stangl, Herbert; Hasegawa, Tomonobu; Richardson, James A.; Parker, Keith L.; Hobbs, Helen H.

In: Molecular Endocrinology, Vol. 13, No. 9, 1999, p. 1460-1473.

Research output: Contribution to journalArticle

Cao, G, Zhao, L, Stangl, H, Hasegawa, T, Richardson, JA, Parker, KL & Hobbs, HH 1999, 'Developmental and hormonal regulation of murine scavenger receptor, class B, type 1', Molecular Endocrinology, vol. 13, no. 9, pp. 1460-1473.
Cao G, Zhao L, Stangl H, Hasegawa T, Richardson JA, Parker KL et al. Developmental and hormonal regulation of murine scavenger receptor, class B, type 1. Molecular Endocrinology. 1999;13(9):1460-1473.
Cao, Guoqing ; Zhao, Liping ; Stangl, Herbert ; Hasegawa, Tomonobu ; Richardson, James A. ; Parker, Keith L. ; Hobbs, Helen H. / Developmental and hormonal regulation of murine scavenger receptor, class B, type 1. In: Molecular Endocrinology. 1999 ; Vol. 13, No. 9. pp. 1460-1473.
@article{588ec85cc7944ce683c5b38c9b38603b,
title = "Developmental and hormonal regulation of murine scavenger receptor, class B, type 1",
abstract = "The scavenger receptor, class B, type I (SR-BI), is the predominant receptor that supplies plasma cholesterol to steroidogenic tissues in rodents. We showed previously that steroidogenic factor-1 (SF-1) binds a sequence in the human SR-BI promoter whose integrity is required for high-level SR-BI expression in cultured adrenocortical tumor cells. We now provide in vivo evidence that SF-1 regulates SR-BI. During mouse embryogenesis, SR-BI mRNA was initially expressed in the genital ridge of both sexes and persisted in the developing testes but not ovary. This sexually dimorphic expression profile of SR-BI expression in the gonads mirrors that of SF-1. No SR-BI mRNA was detected in the gonadal ridge of day 11.5 SF-1 knockout embryos. Both SR-BI and SF-1 mRNA were expressed in the cortical cells of the nascent adrenal glands. These studies directly support SF-1 participating in the regulation of SR-BI in vivo. We examined the effect of cAMP on SR-BI mRNA and protein in mouse adrenocortical (Y1-BS1) and testicular carcinoma Leydig (MA-10) cells. The time courses of induction were strikingly similar to those described for other cAMP- and SF-1-regulated genes. Addition of lipoproteins reduced SR-BI expression in Y1-BS1 cells, an effect that was reversed by administration of cAMP analogs. SR-BI mRNA and protein were expressed at high levels in the adrenal glands of knockout mice lacking the steroidogenic acute regulatory protein; these mice have extensive lipid deposits in the adrenocortical cells and high circulating levels of ACTH. Taken together, these studies suggest that trophic hormones can override the suppressive effect of cholesterol on SR-BI expression, thus ensuring that steroidogenesis is maintained during stress.",
author = "Guoqing Cao and Liping Zhao and Herbert Stangl and Tomonobu Hasegawa and Richardson, {James A.} and Parker, {Keith L.} and Hobbs, {Helen H.}",
year = "1999",
language = "English",
volume = "13",
pages = "1460--1473",
journal = "Molecular Endocrinology",
issn = "0888-8809",
publisher = "The Endocrine Society",
number = "9",

}

TY - JOUR

T1 - Developmental and hormonal regulation of murine scavenger receptor, class B, type 1

AU - Cao, Guoqing

AU - Zhao, Liping

AU - Stangl, Herbert

AU - Hasegawa, Tomonobu

AU - Richardson, James A.

AU - Parker, Keith L.

AU - Hobbs, Helen H.

PY - 1999

Y1 - 1999

N2 - The scavenger receptor, class B, type I (SR-BI), is the predominant receptor that supplies plasma cholesterol to steroidogenic tissues in rodents. We showed previously that steroidogenic factor-1 (SF-1) binds a sequence in the human SR-BI promoter whose integrity is required for high-level SR-BI expression in cultured adrenocortical tumor cells. We now provide in vivo evidence that SF-1 regulates SR-BI. During mouse embryogenesis, SR-BI mRNA was initially expressed in the genital ridge of both sexes and persisted in the developing testes but not ovary. This sexually dimorphic expression profile of SR-BI expression in the gonads mirrors that of SF-1. No SR-BI mRNA was detected in the gonadal ridge of day 11.5 SF-1 knockout embryos. Both SR-BI and SF-1 mRNA were expressed in the cortical cells of the nascent adrenal glands. These studies directly support SF-1 participating in the regulation of SR-BI in vivo. We examined the effect of cAMP on SR-BI mRNA and protein in mouse adrenocortical (Y1-BS1) and testicular carcinoma Leydig (MA-10) cells. The time courses of induction were strikingly similar to those described for other cAMP- and SF-1-regulated genes. Addition of lipoproteins reduced SR-BI expression in Y1-BS1 cells, an effect that was reversed by administration of cAMP analogs. SR-BI mRNA and protein were expressed at high levels in the adrenal glands of knockout mice lacking the steroidogenic acute regulatory protein; these mice have extensive lipid deposits in the adrenocortical cells and high circulating levels of ACTH. Taken together, these studies suggest that trophic hormones can override the suppressive effect of cholesterol on SR-BI expression, thus ensuring that steroidogenesis is maintained during stress.

AB - The scavenger receptor, class B, type I (SR-BI), is the predominant receptor that supplies plasma cholesterol to steroidogenic tissues in rodents. We showed previously that steroidogenic factor-1 (SF-1) binds a sequence in the human SR-BI promoter whose integrity is required for high-level SR-BI expression in cultured adrenocortical tumor cells. We now provide in vivo evidence that SF-1 regulates SR-BI. During mouse embryogenesis, SR-BI mRNA was initially expressed in the genital ridge of both sexes and persisted in the developing testes but not ovary. This sexually dimorphic expression profile of SR-BI expression in the gonads mirrors that of SF-1. No SR-BI mRNA was detected in the gonadal ridge of day 11.5 SF-1 knockout embryos. Both SR-BI and SF-1 mRNA were expressed in the cortical cells of the nascent adrenal glands. These studies directly support SF-1 participating in the regulation of SR-BI in vivo. We examined the effect of cAMP on SR-BI mRNA and protein in mouse adrenocortical (Y1-BS1) and testicular carcinoma Leydig (MA-10) cells. The time courses of induction were strikingly similar to those described for other cAMP- and SF-1-regulated genes. Addition of lipoproteins reduced SR-BI expression in Y1-BS1 cells, an effect that was reversed by administration of cAMP analogs. SR-BI mRNA and protein were expressed at high levels in the adrenal glands of knockout mice lacking the steroidogenic acute regulatory protein; these mice have extensive lipid deposits in the adrenocortical cells and high circulating levels of ACTH. Taken together, these studies suggest that trophic hormones can override the suppressive effect of cholesterol on SR-BI expression, thus ensuring that steroidogenesis is maintained during stress.

UR - http://www.scopus.com/inward/record.url?scp=0033304781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033304781&partnerID=8YFLogxK

M3 - Article

C2 - 10478838

AN - SCOPUS:0033304781

VL - 13

SP - 1460

EP - 1473

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 9

ER -